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Invitrogen™
Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm
Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol.
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| Catalog Number | No. of Wells |
|---|---|
| ZY00100BOX | 10-well |
| ZY00102BOX | 12-well |
| ZY00105BOX | 15-well |
Catalog number ZY00100BOX
Price (MXN)
-
No. of Wells:
10-well
Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.
Formulation
Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and gelatin as the substrate. All have a 4% stacking gel.
Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.
Recommended buffers
Use Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC2675) with these gels. Novex Zymogram Renaturing Buffer (LC2670) and Novex Zymogram Developing Buffer (LC2671) are also recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Gel Percentage0.1
Gel TypeZymogram Gel
Mode of SeparationMolecular Weight
No. of Wells10-well
Quantity10 gels (1 box)
Sample Loading VolumeUp to 25 μL
Separation Range30 to 150 kDa (Tris-Glycine Buffer)
Shipping ConditionWet Ice
Temperature (Metric) StorageStore at 2°C to 8°C. Do not freeze.
Thickness (Metric)1.0 mm
Width (Metric)8 cm
For Use With (Equipment)Mini Gel Tank, XCell SureLock™ Mini-Cell
Gel SizeMini
Product LineNovex
Unit Size10 gels (1 box)