Invitrogen™

Novex™ 10% Zymogram Plus (Gelatin) Protein Gels, 1.0 mm, 10-well

Catalog number:  ZY00100BOX

 Related applications:

Protein Gel Electrophoresis

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Description

Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.

Formulation: Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and casein or gelatin as the substrate. All have a 4% stacking gel.

Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.

Recommended buffers: Use Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer with these gels. Novex Zymogram Renaturing Buffer and Novex Zymogram Developing Buffer are also recommended.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Mini Gel Tank, XCell SureLock™ Mini
Gel Percentage: 10 %
Gel Size: Mini (8 cm x 8 cm)
Gel Type: Zymogram Gel
Mode of Separation: Molecular Weight
Product Line: Novex™
Product Size: 10 gels (1 box)
Quantity: 10 gels
Sample Loading Volume: 25 µL
Separation Range: 30 to 150 kDa (Tris-Glycine Buffer)
Substrate: Gelatin
Substrate Type: Protease Substrate
Thickness: 1 mm
Well Format: 1D Well
Wells: 10 wells
Shipping Condition: Wet Ice

Contents & storage

One box containing 10 gels.
Store in refrigerator (2–8°C). Do not freeze.
Shelf life is 4 months.

Documents

Manuals & protocols