Platinum™ SuperFi™ DNA Polymerase
Platinum™ SuperFi™ DNA Polymerase
Invitrogen™

Platinum™ SuperFi™ DNA Polymerase

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
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Catalog NumberNo. of Reactions
12351010100 Reactions
12351050500 Reactions
123512505 x 500 Reactions
3 Options
Catalog number 12351010
Price (USD)/ 100 units
-
No. of Reactions:
100 Reactions
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Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Benefits of Platinum SuperFi DNA Polymerase

  • Exceptional >300X Taq fidelity
  • High specificity and increased yields with Platinum hot-start technology
  • Robust amplification of difficult targets including those of sub-optimal purity or high GC content
  • Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
  • Amplification of samples with suboptimal purity

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Long PCR

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PolymerasePlatinum SuperFi DNA Polymerase
Reaction SpeedFast
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)300X
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Size (Final Product)20 kb or less
Unit Size100 units
Contents & Storage
• Platinum SuperFi DNA Polymerase (50 μL at 2 U/μL)
• 5X SuperFi Buffer (2 x 1.25 mL)
• 5X SuperFi GC Enhancer (1.25 mL)

Store at –5°C to –30°C.