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Citations & References (6)
Thermo Scientific™
SPDP (succinimidyl 3-(2-pyridyldithio)propionate)
Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavableRead more
| Catalog Number | Quantity |
|---|---|
| 21857 | 50 mg |
Catalog number 21857
Price (USD)
335,34
Each
Quantity:
50 mg
Price (USD)
335,34
Each
Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
Features of SPDP:
• Reactive groups: NHS ester and pyridyldithiol
• Reactive towards: amino and sulfhydryl groups
• Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
• Cleavable crosslinker allows separation of crosslinked products
• Water-insoluble (dissolve first in DMF or DMSO)
• SPDP crosslinker is membrane permeable, so crosslinking can be done inside the cells
• Compare to other varieties of SPDP-type reagents, including pegylated forms
Applications:
• Reversible crosslinking of proteins
• Adding sulfhydryl groups to proteins by attachment to lysine residues
• Protein crosslinking to create antibody-enzyme and other bioconjugates via a two-step reaction
• Attaching haptens to carrier proteins for antibody production
• Preparing immunotoxin conjugates
Specifications:
We manufacture SPDP to the highest specification to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of SPDP is tested to ensure it is >95% pure by quantitative NMR (the highest standard for crosslinker purity).
We offer three analogs of SPDP: the standard version (SPDP), a derivative with a longer spacer arm (LC-SPDP), and a sulfonated water-soluble variety (Sulfo-LC-SPDP). SPDP (N-Succinimidyl 3-(2-pyridyldithio)-propionate) and LC-SPDP (Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate) are heterobifunctional, thiol-cleavable and membrane permeable crosslinkers. They each contain an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
Pierce™ Premium Grade SPDP
LC-SPDP (succinimidyl 6-(3(2-pyridyldithio)propionamido)hexanoate)
Features of SPDP:
• Reactive groups: NHS ester and pyridyldithiol
• Reactive towards: amino and sulfhydryl groups
• Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
• Cleavable crosslinker allows separation of crosslinked products
• Water-insoluble (dissolve first in DMF or DMSO)
• SPDP crosslinker is membrane permeable, so crosslinking can be done inside the cells
• Compare to other varieties of SPDP-type reagents, including pegylated forms
Applications:
• Reversible crosslinking of proteins
• Adding sulfhydryl groups to proteins by attachment to lysine residues
• Protein crosslinking to create antibody-enzyme and other bioconjugates via a two-step reaction
• Attaching haptens to carrier proteins for antibody production
• Preparing immunotoxin conjugates
Specifications:
We manufacture SPDP to the highest specification to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of SPDP is tested to ensure it is >95% pure by quantitative NMR (the highest standard for crosslinker purity).
We offer three analogs of SPDP: the standard version (SPDP), a derivative with a longer spacer arm (LC-SPDP), and a sulfonated water-soluble variety (Sulfo-LC-SPDP). SPDP (N-Succinimidyl 3-(2-pyridyldithio)-propionate) and LC-SPDP (Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate) are heterobifunctional, thiol-cleavable and membrane permeable crosslinkers. They each contain an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
Pierce™ Premium Grade SPDP
LC-SPDP (succinimidyl 6-(3(2-pyridyldithio)propionamido)hexanoate)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityYes
DescriptionSPDP
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)312.36
PEGylatedNo
Product LinePierce
Quantity50 mg
Reactive MoietyNHS Ester, Pyridyldisulfide
Shipping ConditionAmbient
SolubilityDMF, DMSO
Spacer Arm Length6.8 Å
Water SolubleNo
Chemical ReactivityAmine-Sulfhydryl
CleavableBy Thiols
Crosslinker TypeHeterobifunctional
FormatStandard, Premium-grade
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Upon receipt store at -20°C protected from moisture.
Frequently asked questions (FAQs)
Can you provide the shelf-life for SPDP (succinimidyl 3-(2-pyridyldithio)propionate)?
Citations & References (6)
Citations & References
Abstract
Development and characterization of a potent immunoconjugate targeting the Fn14 receptor on solid tumor cells.
Journal:Mol Cancer Ther
PubMed ID:21586630
'TNF-like weak inducer of apoptosis (TWEAK) and fibroblast growth factor (FGF)-inducible 14 (Fn14) are a TNF superfamily ligand-receptor pair involved in many cellular processes including proliferation, migration, differentiation, inflammation, and angiogenesis. The Fn14 receptor is expressed at relatively low levels in normal tissues, but it is known to be dramatically
Evidence for the assembly of a bacterial tripartite multidrug pump with a stoichiometry of 3:6:3.
Journal:J Biol Chem
PubMed ID:21610073
'The multiple transferable resistance (mTR) pump from Neisseria gonorrhoeae MtrCDE multidrug pump is assembled from the inner and outer membrane proteins MtrD and MtrE and the periplasmic membrane fusion protein MtrC. Previously we established that while there is a weak interaction of MtrD and MtrE, MtrC binds with relatively high
Winding single-molecule double-stranded DNA on a nanometer-sized reel.
Journal:Nucleic Acids Res
PubMed ID:22772992
A molecular system of a nanometer-sized reel was developed from F(1)-ATPase, a rotary motor protein. By combination with magnetic tweezers and optical tweezers, single-molecule double-stranded DNA (dsDNA) was wound around the molecular reel. The bending stiffness of dsDNA was determined from the winding tension (0.9-6.0 pN) and the diameter of
Quantitative ratiometric discrimination between noncancerous and cancerous prostate cells based on neuropilin-1 overexpression.
Journal:Proc Natl Acad Sci U S A
PubMed ID:21930955
A multiplexed, ratiometric method is described that can confidently distinguish between cancerous and noncancerous epithelial prostate cells in vitro. The technique is based on bright surface-enhanced resonance Raman scattering (SERRS) biotags (SBTs) infused with unique Raman reporter molecules, and carrying cell-specific peptides. Two sets of SBTs were used. One targets
A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17360572
Bacteria such as Escherichia coli and Pseudomonas aeruginosa expel antibiotics and other inhibitors via tripartite multidrug efflux pumps spanning the inner and outer membranes and the intervening periplasmic space. A key event in pump assembly is the recruitment of an outer membrane-anchored TolC exit duct by the adaptor protein of