MultiScribe™ Reverse Transcriptase
MultiScribe™ Reverse Transcriptase
Citations & References (1)
Invitrogen™

MultiScribe™ Reverse Transcriptase

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.
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Catalog NumberQuantity
4311235100 μL
Catalog number 4311235
Price (USD)
277,02
Each
Add to cart
Quantity:
100 μL
Price (USD)
277,02
Each
Add to cart

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays. MultiScribe Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. This recombinant RNA–dependent, DNA polymerase uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand.

Features of MultiScribe Reverse Transcriptase include:

  • Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay setup
  • Individual component design provides versatility in assay setup
  • 5,000 U is supplied in 100 μL vials (50 U/μL)
  • Reaction temperature up to 52°C
Applications
  • PCR
  • RT-PCR
  • Reverse Transcription
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeFirst-Strand cDNA
FormatStand-alone Enzyme
Optimal Reaction Temperature37°C
Quantity100 μL
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
Size (Final Product)Up to 7 kb
Starting MaterialRNA
TechniqueReverse Transcription
Concentration50 U/μL
For Use With (Application)RT-PCR
Unit SizeEach
Contents & Storage

• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)

Store at –20°C.

Frequently asked questions (FAQs)

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

Can I purchase the MultiScribe Reverse Transcriptase from the TaqMan® MicroRNA Reverse Transcription Kit (Cat. No 4366596) separately?

Yes, the MultiScribe Reverse Transcriptase (Cat. No. 4311235) can be purchased as a standalone product. Please see the following link.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Does Applied Biosystems MultiScribe Reverse Transcriptase have RNase H activity?

Yes, MultiScribe Reverse Transcriptase (Cat. No. 4311235) does have RNase H activity.

What is the difference between MultiScribe Reverse Transcriptase and MuLV Reverse Transcriptase?

MultiScribe Reverse Transcriptase (Cat. No. 4311235) and MuLV Reverse Transcriptase (Cat. No. N808-0018) are the same enzyme, a recombinant Moloney Murine Leukemia Virus (MuLV) Reverse Transcriptase. The only difference is in the recommended usage for each enzyme. MultiScribe Reverse Transcriptase is recommended for use with quantitative nuclease assays, and MuLV is recommended for use in traditional RT-PCR assays.

Citations & References (1)

Citations & References
Abstract
PAMP induced expression of immune relevant genes in head kidney leukocytes of rainbow trout (Oncorhynchus mykiss).
Authors:Chettri JK, Raida MK, Holten-Andersen L, Kania PW, Buchmann K
Journal:Dev Comp Immunol
PubMed ID:21147161
'Host immune responses elicited by invading pathogens depend on recognition of the pathogen by specific receptors present on phagocytic cells. However, the reactions to viral, bacterial, parasitic and fungal pathogens vary according to the pathogen-associated molecular patterns (PAMPs) on the surface of the invader. Phagocytic cells are known to initiate ... More