The original Slide-A-Lyzer™ Dialysis Cassettes will be discontinued once remaining inventory sells out. The next generation of Slide-A-Lyzer™ G3 Dialysis Cassettes offer improved ease of use and are the recommended replacement. Learn more ›
Thermo Scientific™
Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO
Remove salts and contaminants, and maximize sample recovery using this semi-permeable membrane with a 3,500-Dalton protein MW cut-off (MWCO) cassette that is easier to use than traditional dialysis tubing.
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Catalog Number
Sample Volume (Metric)
Quantity
66333
0.5 mL
10 Cassettes
66335
0.5 mL
1 Kit (10 Cassettes)
66330
3 mL
10 Cassettes
66110
12 mL
8 Cassettes
66130
30 mL
6 Cassettes
5 Options
Catalog number 66333
Price (USD)
-
Sample Volume (Metric):
0.5 mL
Quantity:
10 Cassettes
Request bulk or custom format
Slide-A-Lyzer Dialysis Cassettes (3.5K MWCO) facilitate simple and effective removal of salts and small contaminants from proteins and other macromolecules larger than 3,500 Daltons. They can be used for a wide range of applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration.
Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips, or caps are needed to seal the units and prevent leaking and sample loss during the dialysis procedure. Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
Features of Slide-A-Lyzer Dialysis Cassettes: • Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis • Fast dialysis—flat cassette chamber with two membranes provides high surface area-to-volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing • High recovery—rectangular cassette design maximizes recovery of entire sample volume from any one of the four corner injection ports • Four cassette sizes—select the cassette that best suits your sample volume (0.5 to 30 mL) • Color-coded—pink cassette frame specifies cassettes with the 3,500 MW pore size membrane
Membrane specifications: • Composition: regenerated cellulose synthesized by the viscose method • Thickness: 0.9 to 1.2 mil (22.5 to 30 μm) • Hydration time required before use: 2 minutes • Glycerol content: none • Sulfur content: 0.1–0.15% • Heavy metals content: trace
Volume ranges: • 0.5 mL = 0.1 to 0.5 mL • 3 mL = 0.5 to 3 mL • 12 mL = 3 to 12 mL • 30 mL = 12 to 30 mL
For Research Use Only. Not for use in diagnostic procedures.
Specifications
MembraneCellulose Membrane, Dialysis
Product LineSlide-A-Lyzer
Purification TargetBuffer Exchange, Protein
Quantity10 Cassettes
FormatCassette
MWCO3.5 kDa
Sample Volume (Metric)0.5 mL
Unit SizeEach
Contents & Storage
Store at room temperature
Frequently asked questions (FAQs)
Does the Slide-A-Lyzer Dialysis Cassette leak?
The innovative, patented design does not permit wicking that can occur with homemade microdialysis devices.
What sizes are available for the Slide-A-Lyzer Dialysis Cassette? What MWCO membranes are available?
Four sizes to accommodate samples between 0.1 and 30 ml are currently available in five different MWCOs (2K, 3.5K, 7K, 10K and 20K).
The G2 Slide A lyzers on the other hand are available in five different sizes to accommodate samples between 0.25 and 70mL.
These units, especially those that accommodate smaller volumes, provide for greater sample recovery and shorter dialysis times than one can achieve with traditional tubing. We have a cassette selection guide to help you choose the appropriate cassette for your application. There are also product demonstrations for this and other High-Performance Dialysis Products.
What prevents the Slide-A-Lyzer Dialysis Cassette from leaking?
An internal gasket, which isn't visible after the cassette is assembled, reseals itself as the needle is withdrawn. To maintain sample volume and integrity, users should remember these three key rules:
1. Before use, fill the cassette with air to verify that the system is airtight.
2. Puncture each porthole only once.
3. Do not allow the membrane to come into contact with the sharp needle bevel.
What is the purpose of the buoy for the Slide-A-Lyzer Dialysis Cassette?
The buoy suspends the Slide-A-Lyzer Cassette above the stirring bar during dialysis and it provides a benchtop stand on which to set the Slide-A-Lyzer Cassette. These are not required with the G2 Slide-A-Lyzer Dialysis Cassettes.
What is the highest temperature and the pH range that is compatible with the Slide-A-Lyzer Cassette?
Slide-A-Lyzer Cassettes can be held at 56 degrees C for two hours. At temperatures and times above these, the plastic gets soft and can deform. Thermo Scientific recommends that users maintain a pH range of 5-9.