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Citations & References (11)
Invitrogen™
7-Amino-4-Methylcoumarin, reference standard
7-Amino-4-methylcoumarin (AMC) can be used as a fluorescence reference standard for AMC-based enzyme substrates including AMC-based caspase substrates. The fluorescenceRead more
| Catalog Number | Quantity |
|---|---|
| A191 | 100 mg |
Catalog number A191
Price (USD)
273,78
Each
Quantity:
100 mg
Price (USD)
273,78
Each
7-Amino-4-methylcoumarin (AMC) can be used as a fluorescence reference standard for AMC-based enzyme substrates including AMC-based caspase substrates. The fluorescence spectra of AMC is not subject to variability due to pH-dependent protonation/deprotonation when assayed near or above physiological pH.
To make a concentrated stock solution of up to 10 mg/mL, dissolve in either acetone, high quality anhydrous DMSO or DMF. Store frozen, desiccated and protected from light.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Emission430 nm
Excitation351 nm
Label or DyeMethylcoumarin
Molecular Weight (g/mol)175.2
Product Type7-Amino-4-Methylcoumarin
Quantity100 mg
Shipping ConditionRoom Temperature
Label TypeCoumarin Dyes
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
Frequently asked questions (FAQs)
What solvent should I use to reconstitute 7-Amino-4-Methylcoumarin, reference standard (Cat. No. A191)? Once reconstituted, can the product be aliquoted and stored at -20 degrees C without damaging the molecule?
Citations & References (11)
Citations & References
Abstract
High-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography of glycosphingolipids.
Journal:Anal Biochem
PubMed ID:2327579
'A method combining high-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography is introduced for determination of glycosphingolipids. Glycolipids in crude extract from rat liver were separated quantitatively from neutral lipids and phospholipids with a phenylboronic acid-derivatized silica gel column. Glycolipids were eluted quantitatively with approximately 98%
Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay.
Journal:Anal Biochem
PubMed ID:2339787
'A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubation period of between 1 and 16 h, was
New fluorogenic substrates for subtilisin.
Journal:Chem Pharm Bull (Tokyo)
PubMed ID:3899387
A new fluorescent tag for labeling of saccharides.
Journal:Anal Biochem
PubMed ID:6846798
Potential aldehyde groups of N-acetylglucosamine and its di- and tri-saccharides were coupled with 7-amino-4-methylcoumarin (AMC) by reductive amination in the presence of sodium cyanoborohydride. The products were purified by silica gel chromatography. Acid hydrolysis and digestion with glycosidases gave the expected products. One picomole of the sugar-AMC complexes could be
A new fluorogenic substrate for plasmin.
Journal:Biochem J
PubMed ID:161507
A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of