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Fetal Bovine Serum, qualified, USDA-approved regions
Gibco™

Fetal Bovine Serum, qualified, USDA-approved regions

Important Update: We've recently introduced Value Fetal Bovine Serum (FBS) specification-based products. These improved products are alternatives to our Qualified USDA origin and Brazil (South American) origin SKUs. Product stability, performance, manufacturing, storage, and transport processes are unchanged.
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Catalog NumberQuantityFormat
A316060150 mLOne Shot
A316060210 x 50 mLOne Shot
Our improved Value Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Catalog number A3160601

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Replacing with Fetal Bovine Serum, value
  • Sera application: for cell culture with robust cell lines, good value for basic research
  • Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
  • Hemoglobin level: ≤25 mg/dL
  • Origin: countries meeting USDA importation requirements

Gibco serum delivers

  • ISO 13485 certified, processed in FDA registered facilities
  • Triple filtered at 0.1 μm
  • Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
DescriptionFetal Bovine Serum, qualified, One Shot™ format, USDA-approved regions
Product LineOne Shot
Purity or Quality GradeQualified
Shipping ConditionFrozen
SpeciesBovine
AgeFetal
Country of OriginMexico
FormLiquid
FormatOne Shot
Product TypeFetal Bovine Serum
Quantity50 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

Why would I heat inactivate serum?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat inactivated serum is recommended include immunological studies and culturing ES cells, insect cells, and smooth muscle cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I heat inactivate serum?

Heat at 56 degrees C in a water bath for 30 minutes. Swirl bottle every 10 minutes or so. For accuracy, use a second bottle of similar size and add the equivalent volume of water to this bottle. Place a thermometer in this control bottle in order to see when 56 degrees C is reached. Set your timer for 30 minutes at this point.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the USDA Facility Approval Number for Fetal Bovine Serum, qualified, USDA-approved regions?

The Grand Island, NY facility has been assigned facility number NY-TEC-0001 by the USDA.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Has Fetal Bovine Serum, qualified, USDA-approved regions (Cat. Nos. 10437010, 10437028, 10437036, A3160601, A3160602) been heat inactivated? Do I need to heat inactivate it?

Fetal Bovine Serum, qualified, USDA-approved regions (Cat. Nos. 10437010, 10437028, 10437036, A3160601, A3160602) has not been heat inactivated. We would only recommend heat-inactivating it if your application specifically requires it. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, qualified, USDA-approved regions FAQs