Floor Plate Cell Expansion Kit
Floor Plate Cell Expansion Kit
Gibco™

Floor Plate Cell Expansion Kit

The Floor Plate Cell Expansion Kit is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit that is alsoRead more
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Catalog NumberQuantity
A31658011 kit
Catalog number A3165801
Price (USD)
-
Quantity:
1 kit
The Floor Plate Cell Expansion Kit is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit that is also offered separately. It enables additional expansion of floor plate progenitor cells generated using complete Floor Plate Cell Specification Medium (part of the Differentiation kit).

Note: Each additional expansion beyond passage 2 will result in an additional 4–6-fold increase in the total number of floor plate cells without compromising the quality of TH neurons (positive for both TH and FOXA2). Further expansion (up to passage 10) results in stable expansion of progenitor cells, but the resulting TH neurons may show decreased FOXA2 expression.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeStem Cells (Neural)
Shipping ConditionDry ice and gel pack
Quantity1 kit
Unit SizeEach
Contents & Storage
• 1 x 500 mL Floor Plate Cell Expansion Base
• 1 x 10 mL Floor Plate Cell Expansion Supplement

Frequently asked questions (FAQs)

Is cell density in Floor Plate Specification medium critical for Dopaminergic neuron differentiation?

Yes, cell density should be optimized to attain full confluency at day 7 in the specification medium to have an efficient differentiation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Dopaminergic Neuron Maturation medium be used to differentiate neural stem cells?

We have tested neural stem cells (NSCs) isolated from fetal tissue or derived from pluripotent stem cells (PSCs), and have seen that both populations can benefit from maturation medium (DMEM/F12 + Dopaminergic Neuron Maturation Supplement) to have nicely spread homogenous neurons with reduced progenitor population. Matured neurons can be further maintained in neurobasal medium supplemented with Dopaminergic Neuron Maturation Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer Dopaminergic Neuron differentiation service?

Yes. Please go here (https://www.thermofisher.com/us/en/home/life-science/stem-cell-research/stem-cell-services/stem-cell-differentiation-services.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.