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Citations & References (6)
Invitrogen™
mirVana™ miRNA Isolation Kit, without phenol
The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficientRead more
| Catalog Number | Quantity |
|---|---|
| AM1561 | 40 Preps |
Catalog number AM1561
Price (USD)
604,94
Each
Quantity:
40 Preps
Price (USD)
604,94
Each
The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions. Phenol must be purchased separately. Features of this kit:
• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (less than 200 nt) to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types
Efficient recovery of small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana™ miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. The mirVana™ miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 102–107 cultured cells or 0.5–250 mg tissue.
Enrich for small RNA
Although the mirVana™ miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana™ miRNA Isolation Kit, RNA molecules of ∼200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using the mirVana™ miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.
Accessory product
The mirVana™ miRNA Isolation Kit is available for those who want a complete kit that includes the Acid Phenol:Chloroform.
• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (less than 200 nt) to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types
Efficient recovery of small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana™ miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. The mirVana™ miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 102–107 cultured cells or 0.5–250 mg tissue.
Enrich for small RNA
Although the mirVana™ miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana™ miRNA Isolation Kit, RNA molecules of ∼200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using the mirVana™ miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.
Accessory product
The mirVana™ miRNA Isolation Kit is available for those who want a complete kit that includes the Acid Phenol:Chloroform.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume100 μL
Final Product TypeSmall RNA
For Use With (Application)qRT-PCR, microarray analysis, Northern blotting
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time30 min. (After sample homogenization)
Quantity40 Preps
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤107 cells
Cells: ≤107
Plant: ≤250 mg
Tissue: ≤250 mg
Virus: ≤107 cells
Yeast: ≤107 cells
Enzymatic Reactions: varies
Cells: ≤107
Plant: ≤250 mg
Tissue: ≤250 mg
Virus: ≤107 cells
Yeast: ≤107 cells
Enzymatic Reactions: varies
YieldCells: ≤16 μg RNA per 50,000 cells
Tissue: ≤160 μg per 5 mg
Tissue: ≤160 μg per 5 mg
Isolation TechnologySpin Column (Glass Fiber Filter), Organic Extraction
Sample TypeBacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
Unit SizeEach
Contents & Storage
• 30 mL miRNA Wash Solution I (room temperature)
• 50 mL Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 100 mL Lysis/Binding Buffer (4°C)
• 10 mL miRNA Homogenate Additive (4°C)
• 1.4 mL Gel Loading Buffer II (-20°C)
• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
• 50 mL Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 100 mL Lysis/Binding Buffer (4°C)
• 10 mL miRNA Homogenate Additive (4°C)
• 1.4 mL Gel Loading Buffer II (-20°C)
• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Frequently asked questions (FAQs)
What are different methods for cleaning up my miRNA sample?
Can I order separately the Spin Cartridges and the Elution Tubes from the mirVana miRNA Isolation Kit, without phenol (Cat. No. AM1561)?
Do you offer a stand-alone phenol product that I can use with mirVana miRNA Isolation Kit, without phenol (Cat. No. AM1561)?
What kit do you recommend to capture small RNA that is >17 nt in size?
Citations & References (6)
Citations & References
Abstract
[Differential expression profile of microRNA between hyperplastic scar and normal skin].
Journal:Zhonghua Yi Xue Za Zhi
PubMed ID:22781298
To explore the miRNA differential expression profiles of hyperplastic scar and normal skin so as to further elucidate the pathogenesis of hyperplastic scar and search for new therapeutic targets. The total RNA was extracted from 5 human hyperplastic scar and normal skin tissues by Trizol. The specimens were collected from
Circulating microRNAs in serum: novel biomarkers for patients with bladder cancer?
Journal:World J Urol
PubMed ID:23266581
'PURPOSE: Recent studies indicate that circulating microRNAs in serum/plasma are a novel class of non-invasive biomarkers with diagnostic and prognostic information. So far, circulating microRNAs have not been analyzed in patients with bladder cancer. METHODS: We collected serum from patients with non-muscle invasive bladder cancer (NMIBC), muscle invasive bladder cancer
[Isolation, identification and analysis of the expression profile of miRNAs in Aedes albopictus].
Journal:Nan Fang Yi Ke Da Xue Xue Bao
PubMed ID:20423824
'To verify the miRNA in Aedes albopictus and characterize the expression profile of several miRNAs across all the life stages of Aedes albopictus. Based on the published miRNA sequences of Anopheles gambiae and Aedes aegypti, 6 DIG-labeled antisense probes were synthesized. The total RNAs from Aedes albopictus in 6 developmental
MicroRNA expression profiles in the progression of prostate cancer-from high-grade prostate intraepithelial neoplasia to metastasis.
Journal:Urol Oncol
PubMed ID:21880514
'Models of the multistep process related to cancer progression have been designed for many cancers including prostate. The aim of this study is to propose a new model including a possible role for recently described micro RNAs in prostate cancer (CaP) progression. Sixty-three patients underwent radical prostatectomy to treat localized
A model for data analysis of microRNA expression in forensic body fluid identification.
Journal:Forensic Sci Int Genet
PubMed ID:21903498
MicroRNAs (miRNAs, 18-25 bases in length) are small, non-coding RNAs that regulate gene expression at the post-transcriptional level. MiRNA expression patterns, including presence and relative abundance of particular miRNA species, provide cell- and tissue-specific information that can be used for body fluid identification. Recently, two published studies reported that a