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Citations & References (7)
Invitrogen™
CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptoticRead more
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| Catalog Number | Quantity |
|---|---|
| C10740 | 20 Assays |
| C10427 | 100 Assays |
Catalog number C10740
Price (USD)
231,66
Each
Quantity:
20 Assays
Price (USD)
231,66
Each
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.
• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers
CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ∼511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.
Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/EmissionSYTOX AADvanced: 546/647
CellEvent Caspase 3/7 Green Detection Reagent: 511/533
CellEvent Caspase 3/7 Green Detection Reagent: 511/533
Flow Cytometer Laser Lines488
For Use With (Application)Flow Cytometry
For Use With (Equipment)Attune™ Acoustic Focusing Cytometer, Flow Cytometer
Label TypeOther Label(s) or Dye(s)
Label or DyeCellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain
Product LineCellEvent
Product TypeCaspase Assay Kit
Quantity20 Assays
Shipping ConditionWet Ice
Detection MethodFluorescence
FormatKit
Unit SizeEach
Contents & Storage
Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO.
Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light.
Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light.
Frequently asked questions (FAQs)
Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?
With the CellEvent Caspase-3/7 Green Detection Reagent, may I stain cells with nucleic acid stains?
With the CellEvent Caspase-3/7 Green Detection Reagent, may I multiplex with fluorescent immunolabeling?
Is the CellEvent Caspase-3/7 Green Detection Reagent fixable?
Can I fix my CellEvent Caspase 3/7 - labeled cells?
Citations & References (7)
Citations & References
Abstract
Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study.
Journal:Stem Cells Int
PubMed ID:27022399
'Complications of microocclusions have been reported after intra-arterial delivery of mesenchymal stromal cells. Hence, quantification and efficient limitation of cell clumps in suspension before transplantation is important to reduce the risk. We used a flow cytometry-based pulse-width assay to assess the effects of different cell suspension concentrations (0.2-2.0 × 10(6)/mL),
New flow cytometry approaches in equine andrology.
Journal:Theriogenology
PubMed ID:27160445
Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the
Avenanthramide-C reduces the viability of MDA-MB-231 breast cancer cells through an apoptotic mechanism.
Journal:Cancer Cell Int
PubMed ID:29075150
Avenanthramides (AVN) are a relatively unstudied family of phytochemicals that could be novel chemotherapeutics. These compounds, found in oats, are non-toxic to healthy cells and have been shown to reduce viability of human colon and liver cancers in vitro. However, these studies do not elucidate a molecular mechanism for individual
Comparison of
Journal:Anticancer Res
PubMed ID:29061820
The oxazaphosphorines, ifosfamide and cyclophosphamide, represent a class of alkylating agents. The aim of the present in vitro study was to compare antileukemic activity of 4-hydroperoxyifosfamide (4-OOH-IF) and 4-hydroperoxycyclophosphamide (4-OOH-CP).
Liposomal encapsulation of silver nanoparticles enhances cytotoxicity and causes induction of reactive oxygen species-independent apoptosis.
Journal:J Appl Toxicol
PubMed ID:29181855
Silver nanoparticles (AgNP) are one of the most widely investigated metallic NPs due to their promising antibacterial activities. In recent years, AgNP research has shifted beyond antimicrobial use to potential applications in the medical arena. This shift coupled with the extensive commercial applications of AgNP will further increase human exposure