50 mL Tube Magnetic Separator

This Magnetic Separator is for use with the GeneCatcher™ gDNA 3 - 10 ml Blood Kit. The Magnetic Separator consistsRead more

Catalog Number	Quantity
CS15050	1 Each

Catalog number CS15050

Price (USD)

Quantity:

1 Each

This Magnetic Separator is for use with the GeneCatcher™ gDNA 3 - 10 ml Blood Kit. The Magnetic Separator consists of a rack that holds 4 50-ml tubes in alignment with neodymium magnets for the efficient processing of DNA bound to magnetic beads.

GeneCatcher™ Technology is a novel magnetic bead-based technology that provides a method for reliably and efficiently purifying DNA without any centrifugation steps. The GeneCatcher™ gDNA Blood Kits facilitate the efficient and reliable extraction of genomic DNA (gDNA) from samples of human blood, even when the samples have been archived, poorly stored, or collected in the presence of anticoagulants such as EDTA, heparin, or citrate.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)MagnaRack™ Magnetic Separator, Magna-Sep™ Magnetic Particle Separator

Quantity1 Each

Shipping ConditionRoom Temperature

Capacity (Metric)50 mL

Product LineMagnaRack

TypeMagnetic Separator

Unit SizeEach

Contents & Storage

Contains one Magnetic Separator, which may be stored at room temperature.

Frequently asked questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.