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Citations & References (6)
Invitrogen™
Cell Extraction Buffer
Cell Extraction Buffer is a ready-to-use lysis buffer for use in ELISA and western blotting applications for total protein extractionRead more
| Catalog Number | Quantity |
|---|---|
| FNN0011 | 100 ml |
Catalog number FNN0011
Price (USD)
294,84
Each
Quantity:
100 ml
Price (USD)
294,84
Each
Cell Extraction Buffer is a ready-to-use lysis buffer for use in ELISA and western blotting applications for total protein extraction from mammalian cells. The buffer uses detergent-based lysis, eliminating the need for mechanical cell disruption, providing a milder and easier alternative when isolating proteins from cell cultures. The formulation helps retain the protein structure and function needed for enzyme assays or immunoassays.
Buffer formulation: 10 mM Tris, pH 7.4, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 20 mM Na4P2O7, 2 mM Na3VO4, 1% Triton X-100, 10% glycerol, 0.1% SDS, 0.5% deoxycholate
This cell extraction buffer does not contain protease inhibitors and should be supplemented with 1 mM PMSF and protease inhibitor cocktail just prior to use.
View all available ELISA lysis buffers and reagents.
Buffer formulation: 10 mM Tris, pH 7.4, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 20 mM Na4P2O7, 2 mM Na3VO4, 1% Triton X-100, 10% glycerol, 0.1% SDS, 0.5% deoxycholate
This cell extraction buffer does not contain protease inhibitors and should be supplemented with 1 mM PMSF and protease inhibitor cocktail just prior to use.
View all available ELISA lysis buffers and reagents.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Western Blot, Sample preparation for ELISA
Product TypeCell Extraction Buffer
Quantity100 ml
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).
Frequently asked questions (FAQs)
What extraction reagents are recommended for efficient mouse tissue analysis?
During my ProcartaPlex assay data analysis, I am getting a warning message that there is high bead aggregation. What should I do?
During my ProcartaPlex assay data analysis, the beads fall below or to the lower left of their bead region on the bead map. Why is this?
During my ProcartaPlex assay data analysis, beads do not appear in the region gated. What happened?
The bead counts for all of my ProcartaPlex assay wells are erratic. What went wrong?
Citations & References (6)
Citations & References
Abstract
Imaging EGFR phosphorylation in intact human pancreatic carcinoma cells.
Journal:In Vivo
PubMed ID:22773576
'Carcinoma of the pancreatic duct is a highly malignant tumor characterized by aggressive and early metastastatic growth. A high rate of tumor recurrence after surgical resection and the lack of effective chemotherapeutic approaches result in low 5-year survival rates. Overexpression of epidermal growth factor (EGF) and its receptor have been
Induction of apoptosis pathways in several cell lines following exposure to the marine algal toxin azaspiracid.
Journal:Chem Res Toxicol
PubMed ID:22725096
'Azaspiracids (AZAs) are polyether marine dinoflagellate toxins that accumulate in shellfish and represent an emerging human health risk. Although there have been no deaths associated with the AZA toxins, humans exposed to AZAs experience severe gastrointestinal symptoms. This toxin class has been shown to be highly cytotoxic, a teratogen to
Subtoxic levels hydrogen peroxide-induced production of interleukin-6 by retinal pigment epithelial cells.
Journal:Mol Vis
PubMed ID:21031020
To study the effect of subtoxic levels of hydrogen peroxide (H(2)O(2)) on the expression and release of interleukin-6 (IL-6) by cultured retinal pigment epithelial (RPE) cells and to explore the relevant signal pathways.
Interleukin-1beta increases baseline expression and secretion of interleukin-6 by human uveal melanocytes in vitro via the p38 MAPK/NF-kappaB pathway.
Journal:Invest Ophthalmol Vis Sci
PubMed ID:21398280
Melanocyte is the major cell component in the uvea. Interleukin (IL)-6 is a proinflammatory cytokine. The authors studied the expression of IL-6 in cultured human uveal melanocytes (UMs) and their modulation by IL-1ß and explored the relevant signal pathways.
Twist modulates human trophoblastic cell invasion via regulation of N-cadherin.
Journal:Endocrinology
PubMed ID:22166980
The invasion of extravillous cytotrophoblasts (EVT) into the underlying maternal tissues and vasculature is a key step in human placentation. The molecular mechanisms involved in the development of the invasive phenotype of EVT include many that were first discovered for their role in cancer cell metastasis. Previous studies have demonstrated