Rat Primary Cortical Astrocytes
Rat Primary Cortical Astrocytes
Gibco™

Rat Primary Cortical Astrocytes

Gibco Rat Primary Cortical Astrocytes provide neuroscience researchers with a ready-to-use, highly pure source of astrocytes for experiments.
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Catalog NumberQuantity
N7745100
also known as N7745-100
1 x 106 cells
Catalog number N7745100
also known as N7745-100
Price (USD)
-
Quantity:
1 x 106 cells

Gibco Rat Primary Cortical Astrocytes are isolated and cryopreserved from the cortex of Sprague Dawley rats at day E19 of gestation. The astrocytes are passaged only once before cryopreservation and consistently yield post-thaw viability of 70% or greater. Each vial contains 1 million cells per mL and provides a flexible, ready-to-use, and quality alternative to fresh astrocytes.

Rat Primary Cortical Astrocyte details

  • Exhibit ≥70% viability upon thawing
  • Stain >80% positive for the astrocyte specific marker glial fibrillary acid protein (GFAP)
  • Stain ≤10% positive for neuron and oligodendrocyte specific markers galactocerebroside (GalC) and doublecortin (DCX) or tubulin beta-3 (TUBB3)
  • Exhibit a doubling time of approximately nine days at P2
  • Can be expanded in culture for at least one passage
For Research Use Only. Not for use in diagnostic procedures.
Specifications
No. of Cells1 x 106 cells
Product TypeRat Cortical Astrocyte
Quantity1 x 106 cells
Tested ForCell Purity, Sterility, Viability, Mycoplasma
AgeFetal
Cell TypeGlial Cells
Culture TypeMammalian Cell Culture
FormCryopreserved
SpeciesRat
TissueCortex
Unit SizeEach
Contents & Storage
Store in liquid nitrogen, vapor-phase.

Frequently asked questions (FAQs)

What are some of the key applications for astrocytes?

Astrocytes can be used to research cell transplantation, intracellular transport, ion-channel function, toxicity, receptor signaling, analyses of electrophysiological properties, neurotransmitter release, and evaluation of interaction between neurons and astrocytes.

I used rat primary cortical astrocytes from another company and observed cell aggregates after thawing but not with the astrocytes from your company. Why?

Gibco Rat Primary Cortical Astrocytes were dissociated into single cells before cryopreservation, which gives an advantage to control the number of cells to plate.

How can I coat Gibco Rat Primary Cortical Astrocytes on glass coverslips?

We recommend cleaning the coverslips and then coating them with 0.01% poly-D-lysine in double distilled water.

What is the doubling time of Gibco Rat Primary Cortical Astrocytes?

The doubling time of Gibco Rat Primary Cortical Astrocytes is about 8.7 days.

For how many passages can I expand Gibco Rat Primary Cortical Astrocytes?

Gibco Rat Primary Cortical Astrocytes can be thawed, recovered, and proliferated up to three passages to yield 1.5-2 fold increase of cells.

View all Rat Primary Cortical Astrocytes FAQs