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Citations & References (22)
Invitrogen™
Quant-it™ RiboGreen Reagent and RNA Assay Kit
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
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| Catalog Number | Quantity | Product Type | Quantitation Range |
|---|---|---|---|
| R11491 | 1 mL | Quant-iT RiboGreen RNA Reagent | 1 to 200 ng |
| R11490 | 1 kit | Quant-iT RiboGreen RNA Kit | 1 to 200 ng |
Catalog number R11491
Price (USD)
1.137,24
Each
Quantity:
1 mL
Product Type:
Quant-iT RiboGreen RNA Reagent
Quantitation Range:
1 to 200 ng
Price (USD)
1.137,24
Each
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
Workflow
This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection. To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL. RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.
Applications
Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200-2,000 cuvette assays using a 2 mL assay volume
Product LineQuant-iT, RIBOGREEN
Product TypeQuant-iT RiboGreen RNA Reagent
Quantitation Range1 to 200 ng
Quantity1 mL
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach
Frequently asked questions (FAQs)
Why am I getting negative fluorescence values with my Qubit Assays?
What are the linear ranges of the Quant-iT RNA kits?
What is the difference between the Quant-iT RNA assay and the Quant-iT RiboGreen RNA Assay?
Can I make my own assay for the Qubit Fluorometer?
I have a crude lysate. Will the Quant-iT and Qubit assays work?
Citations & References (22)
Citations & References
Abstract
A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases.
Journal:Anal Biochem
PubMed ID:14690681
The properties of DNA-dependent RNA polymerases have been studied since the 1960s, but considerable interest in probing RNA polymerase structure/function relationships, the roles of different classes of RNA polymerases in cellular processes, and the feasibility of using RNA polymerases as drug targets still exists. Historically, RNA polymerase activity has been
Quantification of enterovirus RNA in sludge samples using single tube real-time RT-PCR.
Journal:Biotechniques
PubMed ID:10907082
'We have developed a quantitative RT-PCR method that can be used to determine the amount of enterovirus RNA in urban sludge samples. This method combines Taq-Man technology with the ABI Prism 7700 real-time sequence detection system. We optimized a one-step RT-PCR that uses a dual-labeled fluorogenic probe to quantify the
mRNA expression profiling of laser microbeam microdissected cells from slender embryonic structures.
Journal:Am J Pathol
PubMed ID:11891179
'Microarray hybridization has rapidly evolved as an important tool for genomic studies and studies of gene regulation at the transcriptome level. Expression profiles from homogenous samples such as yeast and mammalian cell cultures are currently extending our understanding of biology, whereas analyses of multicellular organisms are more difficult because of
A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
Journal:J Biol Chem
PubMed ID:12840024
'The Nek family of protein kinases in humans is composed of 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. Nek6 (314AA) and Nek7 (303AA), 76% identical, have little
Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization.
Journal:Biotechniques
PubMed ID:16925017