Suero bovino fetal (SFB), Australia
Suero bovino fetal (SFB), Australia
Gibco™

Suero bovino fetal (SFB), Australia

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Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información
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Número de catálogoCantidadFormatoGrado de pureza o calidad
10099141C500 mLFrascoCualificado
10099141500 mLFrascoCualificado
A316100150 mLOne ShotCualificado
A3161001C50 mLOne ShotCualificado
A316100210 x 50 mLOne ShotCualificado
A3161002C10 × 50 mLOne ShotCualificado
Número de catálogo 10099141C
Precio (USD)
-
Cantidad:
500 mL
Formato:
Frasco
Grado de pureza o calidad:
Cualificado
Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto

Categoría de los sueros: Premium (Secure)
Origen : Australia.
Nivel de endotoxinas: ≤5 EU/ml.
Nivel de hemoglobina: ≤30 mg/dl (niveles habituales ≤25 mg/dl).
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Concentración de endotoxinas≤5 EU/mL
Concentración de hemoglobina≤30 mg/dL
Grado de pureza o calidadCualificado
Condiciones de envíoFrozen
EspecieCattle/Bovine
AgeFetal
País de origenAustralia
FormatoFrasco
Tipo de productoFetal Bovine Serum
Cantidad500 mL
Serum TreatmentStandard (Sterile-filtered)
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Storage conditions: -5°C to -20°C
Shipping conditions: Frozen

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.