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Invitrogen™

Platinum™ Taq DNA Polymerase, Brasil

Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
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Número de catálogoN.º de reacciones
10966020100 reacciones
10966030500 reacciones
109661745000 reacciones
Número de catálogo 10966020
Precio (USD)
75,60
Each
N.º de reacciones:
100 reacciones
Precio (USD)
75,60
Each
Platinum Taq DNA Polymerase is a convenient and reliable hot start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity (but not 3' to 5' exonuclease activity). PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

  • Assembly of PCR reactions at room temperature
  • Hot-start kinetics reduce nonspecific primer annealing and improve product yield
  • May be used for PCR products up to 10 kb
  • Optional KB Extender provides more versatility in PCR assays

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Fidelidad (frente a Taq)1X
Inicio en calienteBuilt-In Hot Start
N.º de reacciones100 reacciones
Sobrante3'-A
PolimerasaPlatinum Taq DNA Polymerase
Cantidad100 reactions
Condiciones de envíoDry Ice
Tamaño (producto final)10 kb or less
Material de partidaADN
Concentración5 U/μL
Para utilizar con (aplicación)Hot-start PCR
GC-Rich PCR PerformanceMedio
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
• Platinum Taq DNA Polymerase, 20 μL
• 10X PCR Buffer (without magnesium), 1.25 mL
• 50 mM MgCl2, 1 mL

Store at -15°C to -25°C in a non-frost-free freezer.

Preguntas frecuentes

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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