Taq DNA Polymerase (Brasil)
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Invitrogen™

Taq DNA Polymerase (Brasil)

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa.
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Número de catálogoN.º de reacciones
11615010500 reacciones
116150445000 reacciones
Número de catálogo 11615010
Precio (USD)
91,39
Each
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N.º de reacciones:
500 reacciones
Precio (USD)
91,39
Each
Añadir al carro de la compra
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity (see figure).

Taq DNA Polymerase native enzyme is purified from Thermus aquaticus YT1. Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.

Features

  • Choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Fidelidad (frente a Taq)1X
Inicio en calienteNo
N.º de reacciones500 reacciones
Sobrante3'-A
PolimerasaTaq DNA Polymerase
Cantidad500 Units
Condiciones de envíoDry Ice
Tamaño (producto final)5 kb o menos
Material de partidaADN
Concentración5 U/μL
Para utilizar con (aplicación)RT-PCR
GC-Rich PCR PerformanceLow
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
• Taq DNA Polymerase (5 U/μL), 100 μL
• 10X PCR Buffer (without magnesium), 2.5 mL
• 50 mM MgCl2, 1 mL

Store at -20°C

Preguntas frecuentes

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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