Mezcla de nutrientes F-12 de Ham
Mezcla de nutrientes F-12 de Ham
Citas y referencias (5)
Gibco™

Mezcla de nutrientes F-12 de Ham

La mezcla de nutrientes F12 de Ham (F-12) fue diseñada para una placa de célula única libre de suero deMás información
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Número de catálogoCantidad
117650706 x 1000 mL
11765054500 mL
1176506210 × 500 mL
117650471000 mL
Número de catálogo 11765070
Precio (USD)
526,50
Each
Añadir al carro de la compra
Cantidad:
6 x 1000 mL
Customize this product
Precio (USD)
526,50
Each
Añadir al carro de la compra
La mezcla de nutrientes F12 de Ham (F-12) fue diseñada para una placa de célula única libre de suero de células de ovario de hámster chino (CHO). El F-12 ya se ha utilizado para el crecimiento libre de suero de cultivos de CHO además de para el crecimiento complementado con suero de otras células de mamíferos, incluidos condrocitos y células epiteliales de la próstata de rata. Ofrecemos una gran variedad de modificaciones de F-12 para una gama de aplicaciones de cultivos celulares. Busque la formulación adecuada mediante la herramienta de selección de medios.

Este F-12 de Ham se modifica del siguiente modo:
Con
• L-glutamina
• Rojo de fenol

Está disponible la formulación completa.

Uso de F-12
En comparación con otros medios basales, el F-12 contiene una variedad más amplia de componentes, incluidos cinc, putrescina, hipoxantina y timidina. El F-12 no contiene proteínas ni factores de crecimiento. Por lo tanto, el F-12 requiere una suplementación, normalmente con suero fetal bovino (SFB) al 10 %. El F-12 utiliza un sistema de tampón de bicarbonato de sodio (1,176 g/l) y, por lo tanto, requiere un entorno con un 5-10 % de CO2 para mantener el pH fisiológico.

Sistema de fabricación y calidad conforme a las buenas prácticas de fabricación actuales
Para mantener la continuidad de la cadena de suministro, fabricamos F-12 en dos instalaciones separadas; una ubicada en Grand Island, Nueva York, y la otra en Escocia, Reino Unido. Ambos sitios cumplen los requisitos de producción según las buenas prácticas de fabricación actuales, cuentan con la certificación ISO 13485 y están registrados en la FDA como fabricantes de dispositivos médicos.
Especificaciones
Línea de célulasCHO, COS-7, and rat prostate epithelial cells
Tipo de célulaPrimary Rat Astrocytes
Concentración1 X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoHam's F-12 Nutrient Mixture
Cantidad6 x 1000 mL
Duración de almacenamiento12 Months From Date of Manufacture
ClasificaciónAnimal Origin-free
FormularioLíquido
EsterilidadEstéril con filtro
Sterilization MethodSterile-filtered
Con aditivosGlutamine, Phenol Red, Sodium Pyruvate
Sin aditivosNo HEPES
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Mezcla de nutrientes F-12 de Ham FAQs

Citations & References (5)

Citations & References
Abstract
Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.
Authors: Takagi Y; Ninomiya H; Sakamoto A; Miwa S; Masaki T;
Journal:J Biol Chem
PubMed ID:7730310
'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type ... More
Molecular cloning and functional analysis of the promoter of the human squalene synthase gene.
Authors: Guan G; Jiang G; Koch R L; Shechter I;
Journal:J Biol Chem
PubMed ID:7665618
'We have cloned and characterized the 5''-flanking region of the gene encoding human squalene synthase. We report here the promoter activity of successively 5''-truncated sections of a 1 kilobase of this region by fusing it to the coding region of a luciferase reporter gene. DNA segments of 200 base pairs ... More
Functional analysis of the human D2 dopamine receptor missense variants.
Authors: Cravchik A; Sibley D R; Gejman P V;
Journal:J Biol Chem
PubMed ID:8824240
The human dopamine D2 receptor gene (DRD2) has three polymorphic variants that predict the amino acid substitutions Val96 --> Ala, Pro310 --> Ser, and Ser311 --> Cys in the receptor protein. We have investigated the ligand binding and signal transduction properties of these human D2 receptor variants by stably expressing ... More
Requirement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor for selected GH-stimulated function.
Authors: Lobie P E; Allevato G; Nielsen J H; Norstedt G; Billestrup N;
Journal:J Biol Chem
PubMed ID:7665593
We have examined the involvement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor in the cellular response to GH. Stable Chinese hamster ovary (CHO) cell clones expressing a receptor with tyrosine residues at position 333 and 338 of the receptor substituted for phenylalanine (CHO-GHR1-638 Y333F, Y338F) ... More
Mutational analysis of the insulin-like growth factor I prohormone processing site.
Authors: Duguay S J; Lai-Zhang J; Steiner D F;
Journal:J Biol Chem
PubMed ID:7615562
Insulin-like growth factor I (IGF-I) is a mitogenic peptide that is produced in most tissues and cell lines and plays an important role in embryonic development and postnatal growth. IGF-I is initially synthesized as a prohormone precursor that is converted to mature IGF-I by endoproteolytic removal of the carboxyl-terminal E-domain. ... More