Gateway™ pDEST™14 Vector

Citas y referencias (3)

Invitrogen™

Gateway™ pDEST™14 Vector

Para adaptarse a todas sus necesidades de expresión, Invitrogen ofrece vectores de destino Gateway™ de vanguardia de destino para laMás información

Número de catálogo	Cantidad
11801016	6 μg

Número de catálogo 11801016

Precio (USD)

913,68

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Cantidad:

Precio (USD)

913,68

Añadir al carro de la compra

Para adaptarse a todas sus necesidades de expresión, Invitrogen ofrece vectores de destino Gateway™ de vanguardia de destino para la expresión en célula de E. coli, insecto, levadura o mamífero, así como para la producción de proteína nativa o proteínas de fusión N o C-terminal. Los vectores de destino Gateway™ tienen sitios attR para la recombinación con cualquier fragmento flanqueado por attL, independientemente de si se trata de un clon de entrada o de un clon Ultimate™ RF. La siguiente tabla enumera la amplia gama de vectores de destino disponibles.

Materiales adicionales necesarios, disponibles por separado: Clon de entrada Gateway™, mezcla de enzimas Gateway™ LR Clonase™ y tampón de reacción.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Resistencia bacteriana a los antibióticosAmpicilina (AMPR)

Sistema constitutivo o inducibleInducible

Tipo de productoVector de expresión

Cantidad6 μg

Agente de selección (eucariótico)Ninguno

VectorpDEST, vectores Gateway T7

Método de clonaciónGateway

Línea de productosGateway, pDEST

PromotorT7

Etiqueta de proteínaSin etiquetar

Unit SizeEach

Contenido y almacenamiento

Todos los vectores de destino se suministran liofilizados y superenrollados.

Preguntas frecuentes

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™14 Vector FAQs

Citations & References (3)

Citations & References

Abstract

Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein.

Authors: Li Zaibo; Na Xi; Wang Dakun; Schoen Susan R; Messing Edward M; Wu Guan;

Journal:J Biol Chem

PubMed ID:11739384

'von Hippel-Lindau (VHL) disease is a hereditary cancer syndrome caused by germline mutations of the VHL gene. Recent studies suggest that VHL protein (pVHL) is a component of an E3 ubiquitin ligase, but the detailed biological function of pVHL remains to be determined. To further elucidate the biological functions of ... More

Molecular chaperone Hsp90 associates with resistance protein N and its signaling proteins SGT1 and Rar1 to modulate an innate immune response in plants.

Authors:Liu Y, Burch-Smith T, Schiff M, Feng S, Dinesh-Kumar SP,

Journal:J Biol Chem

PubMed ID:14583611

SGT1 and Rar1 are important signaling components of resistance (R) gene-mediated plant innate immune responses. Here we report that SGT1 and Rar1 associate with the molecular chaperone Hsp90. In addition, we show that Hsp90 associates with the resistance protein N that confers resistance to tobacco mosaic virus. This suggests that ... More

Serinc, an activity-regulated protein family, incorporates serine into membrane lipid synthesis.

Authors:Inuzuka M, Hayakawa M, Ingi T,

Journal:J Biol Chem

PubMed ID:16120614

Cell membranes contain various transporter proteins, some of which are responsible for transferring amino acids across membrane. In this study, we report another class of carrier proteins, termed Serinc1-5, that incorporates a polar amino acid serine into membranes and facilitates the synthesis of two serine-derived lipids, phosphatidylserine and sphingolipids. Serinc ... More