Baculovirus Expression System with Gateway™ Technology
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Citas y referencias (3)
Invitrogen™

Baculovirus Expression System with Gateway™ Technology

El sistema de expresión de baculovirus con tecnología Gateway™ está diseñado para crear plásmidos pFastBac™ recombinantes que contengan el promotorMás información
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Número de catálogoCantidad
1182701120 reacciones
Número de catálogo 11827011
Precio (USD)
-
Cantidad:
20 reacciones
El sistema de expresión de baculovirus con tecnología Gateway™ está diseñado para crear plásmidos pFastBac™ recombinantes que contengan el promotor de poliedrina. El sistema de expresión de baculovirus con tecnología Gateway™ ofrece:

• Expresión utilizando la tecnología Bac-to-Bac™ (1)
• Vectores de destino que transportan el promotor de poliedrina para la producción de proteínas nativas (pDEST™8), de fusión de histidina N-terminal (pDEST™10) y de fusión de GST N-terminal (pDEST™20)
• Vector pDEST™10 que contiene un sitio de escisión de proteasas TEV para la eliminación de la etiqueta de fusión después de la purificación de proteínas (2)
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de productoSistema de expresión
Cantidad20 reacciones
VectorpDEST, vectores de baculovirus Gateway
Método de clonaciónGateway
Línea de productosGateway
PromotorPoliedrina
Etiqueta de proteínaEtiqueta GST, Etiqueta His (6x), Sin etiquetar
Unit SizeEach
Contenido y almacenamiento
El sistema de expresión en baculovirus con tecnología Gateway™ incluye mezcla de enzimas LR Clonase™ y tampón de reacción, vectores de destino pDEST™8, pDEST™10 y pDEST™20, solución de proteinasa K (2 μg/μl), control positivo pENTR™-gus y células competentes Library Efficiency™ DH5. El kit no incluye células competentes MAX Efficiency™ DH10Bac™. Almacenar la mezcla enzimática LR Clonase™ y las células competentes a -80°C. Almacenar todos los demás componentes a -20°C. Se garantiza la estabilidad de los componentes durante 6 meses si se almacenan correctamente.

Preguntas frecuentes

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Baculovirus Expression System with Gateway™ Technology FAQs

Citations & References (3)

Citations & References
Abstract
Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.
Authors:Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H,
Journal:J Biol Chem
PubMed ID:14699162
'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the ... More
Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation.
Authors:Suzuki T, Okada Y, Semba S, Orba Y, Yamanouchi S, Endo S, Tanaka S, Fujita T, Kuroda S, Nagashima K, Sawa H,
Journal:J Biol Chem
PubMed ID:15843383
'The human polyomavirus JC virus (JCV) is the causative agent of a fatal demyelinating disease, progressive multifocal leukoencephalopathy, and encodes six major proteins, including agnoprotein. Agnoprotein colocalizes with microtubules in JCV-infected cells, but its function is not fully understood. We have now identified fasciculation and elongation protein zeta 1 (FEZ1) ... More
A novel abetalipoproteinemia genotype. Identification of a missense mutation in the 97-kDa subunit of the microsomal triglyceride transfer protein that prevents complex formation with protein disulfide isomerase.
Authors: Rehberg E F; Samson-Bouma M E; Kienzle B; Blinderman L; Jamil H; Wetterau J R; Aggerbeck L P; Gordon D A;
Journal:J Biol Chem
PubMed ID:8939939
The microsomal triglyceride transfer protein (MTP) is a heterodimer composed of the ubiquitous multifunctional protein, protein disulfide isomerase, and a unique 97-kDa subunit. Mutations that lead to the absence of a functional 97-kDa subunit cause abetalipoproteinemia, an autosomal recessive disease characterized by a defect in the assembly and secretion of ... More