X-Gal
X-Gal
Citas y referencias (1)
Invitrogen™

X-Gal

X-Gal (5-bromo-4-cloro-3-indol-β-D-galactosida) es un sustrato histoquímico para la β-galactosidasa.Este sustrato produce un precipitado azul en la hidrólisis, lo que loMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoCantidadTipo de producto
155200181 gSustrato X-Gal
15520034100 mgSustrato X-Gal
Número de catálogo 15520018
Precio (USD)
573,48
Each
Añadir al carro de la compra
Cantidad:
1 g
Tipo de producto:
Sustrato X-Gal
Precio (USD)
573,48
Each
Añadir al carro de la compra
X-Gal (5-bromo-4-cloro-3-indol-β-D-galactosida) es un sustrato histoquímico para la β-galactosidasa.Este sustrato produce un precipitado azul en la hidrólisis, lo que lo hace adecuado para su uso en inmunotransferencias o ensayos inmunocitoquímicos.Las bacterias que contienen β-galactosidasa activa producen colonias azules cuando se cultivan en placas que contienen X-Gal. X-Gal se suministra como cristales blancos y es soluble en dimetilformamida.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Nombre del producto químico o material5-bromo-4-cloro-3-indolil-β-D-galactosidasa
DescripciónX-Gal, penetra en la célula
Para utilizar con (aplicación)Ensayos inmunocitoquímicos
Tipo de productoSustrato X-Gal
Cantidad1 g
Condiciones de envíoAprobado para su envío a temperatura ambiente o en hielo húmedo
Método de detecciónColorimétrico
FormularioPolvo
FormatoBottle
Unit SizeEach
Contenido y almacenamiento
Contiene 1 g de X-Gal, polvo

Preguntas frecuentes

Can X-gal be resuspended in dimethylsulfoxide (DMSO) instead of dimethylformamide (DMF)? How are X-gal plates prepared for blue/white screening?

X-gal (also known as 5-bromo-4-chloro-3-indolyl-?-D-galactoside) is soluble in either DMSO or DMF, and can be stored in solution in the freezer for up to 6 months. Protect the solution from light.

To add X-gal to previously made agar plates, pipette 40 ?l of a 40 mg/ml stock solution onto a 100mm pre-poured agar plate and spread evenly with a sterile spreader. Let dry 15 minutes and store plates away from light.

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Citations & References (1)

Citations & References
Abstract
Identification of endopeptidase genes from the genomic sequence of Lactobacillus helveticus CNRZ32 and the role of these genes in hydrolysis of model bitter peptides.
Authors:Sridhar VR, Hughes JE, Welker DL, Broadbent JR, Steele JL,
Journal:Appl Environ Microbiol
PubMed ID:15932998
Genes encoding three putative endopeptidases were identified from a draft-quality genome sequence of Lactobacillus helveticus CNRZ32 and designated pepO3, pepF, and pepE2. The ability of cell extracts from Escherichia coli DH5alpha derivatives expressing CNRZ32 endopeptidases PepE, PepE2, PepF, PepO, PepO2, and PepO3 to hydrolyze the model bitter peptides, beta-casein (beta-CN) ... More