Gentamicina (50 mg/ml)
Gentamicina (50 mg/ml)
Citas y referencias (5)
Gibco™

Gentamicina (50 mg/ml)

El sulfato de gentamicina es un antibiótico soluble en agua purificado originalmente a partir del hongo Micromonospora purpurea.La gentamicina actúaMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoCantidad
1575007810 x 10 mL
1575006010 mL
Número de catálogo 15750078
Precio (USD)
1.121,04
Each
Añadir al carro de la compra
Cantidad:
10 x 10 mL
Precio (USD)
1.121,04
Each
Añadir al carro de la compra
El sulfato de gentamicina es un antibiótico soluble en agua purificado originalmente a partir del hongo Micromonospora purpurea.La gentamicina actúa uniéndose a la subunidad 30S del ribosoma bacteriano, lo que conduce a la inhibición de la síntesis de proteínas y a la muerte de las bacterias susceptibles.La gentamicina Gibco™ es eficaz contra una amplia variedad de bacterias grampositivas y gramnegativas, y se utiliza para la prevención de la contaminación bacteriana de cultivos celulares.La concentración de trabajo recomendada oscila entre 0,5 y 50 μg/ml.

Ofrecemos una amplia gama de antibióticos y antimicóticos tanto en polvo como en líquido.

Obtenga más información sobre el uso de antibióticos y antimicóticos en el cultivo celular y revise las directrices para la descontaminación de cultivos.

Fabricación según las prácticas correctas actuales en dos instalaciones
Gibco™ La gentamicina se fabrica en instalaciones que cumplen los principios de buenas prácticas actuales, ubicadas en Grand Island, Nueva York.Las instalaciones están registradas en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485.Para mantener la continuidad de la cadena de suministro, podemos ofrecer un producto de gentamicina Gibco™ comparable que fabricamos en nuestras instalaciones de Escocia (15750-037).Estas instalaciones están registradas en la FDA como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485.
Para uso exclusivo en investigación.No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración50 mg/mL
Almacenamiento recomendadoCondiciones de almacenamiento: De 15 °C a 30 °C
Condiciones de envío: Temperatura ambiente
Vida útil: 24 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
Filtrado estérilYes
Aplicación validadaPrevención de la contaminación del cultivo celular
Forma físicaLíquido
Cantidad10 x 10 mL
TipoGentamicina
Unit SizeEach

Preguntas frecuentes

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (5)

Citations & References
Abstract
Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.
Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,
Journal:J Biol Chem
PubMed ID:22810232
'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
Authors:Avci FY, Li X, Tsuji M, Kasper DL,
Journal:Nat Protoc
PubMed ID:23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More
A three-dimensional co-culture system to investigate macrophage-dependent tumor cell invasion.
Authors:Dwyer AR, Ellies LG, Holme AL, Pixley FJ
Journal:J Biol Methods
PubMed ID:31453214
'Macrophages infiltrate cancers and promote progression to invasion and metastasis. To directly examine tumor-associated macrophages (TAMs) and tumor cells interacting and co-migrating in a three-dimensional (3D) environment, we have developed a co-culture model that uses a PyVmT mouse mammary tumor-derived cell line and mouse bone marrow-derived macrophages (BMM). The Py8119 ... More
Characterizing the Antimicrobial Function of a Dairy-Originated Probiotic,
Authors:Nair DVT, Kollanoor Johny A
Journal:Front Microbiol
PubMed ID:30050507
'Antimicrobial potential of a dairy-origin probiotic bacteria,'
Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells.
Authors:Conroy LR, Lorkiewicz P, He L, Yin X, Zhang X, Rai SN, Clem BF
Journal:Cancer Cell Int
PubMed ID:32624705
Aberrant activity of cell cycle proteins is one of the key somatic events in non-small cell lung cancer (NSCLC) pathogenesis. In most NSCLC cases, the retinoblastoma protein tumor suppressor (RB) becomes inactivated via constitutive phosphorylation by cyclin dependent kinase (CDK) 4/6, leading to uncontrolled cell proliferation. Palbociclib, a small molecule ... More