La agarosa UltraPure™ es un polisacárido utilizado para la separación basada en el tamaño de ácidos nucleicos en aplicaciones deMás información
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Cantidad
16500500
500 g
16500100
100 g
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Número de catálogo 16500500
Precio (USD)
1.013,94
Each
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Cantidad:
500 g
Pedido a granel o personalizado
Precio (USD)
1.013,94
Each
Añadir al carro de la compra
La agarosa UltraPure™ es un polisacárido utilizado para la separación basada en el tamaño de ácidos nucleicos en aplicaciones de electroforesis en gel de agarosa. La agarosa UltraPure™ es ideal para resolver fragmentos de ADN y ARN de 100 bp a > 30 kb. Características de la agarosa UltraPure™:
• Ideal para el análisis y la recuperación de ADN y ARN para aplicaciones rutinarias • Fuerte estructura de gel que permite una mejor manipulación y menos roturas • Se puede utilizar en aplicaciones de electroforesis de proteínas, como pruebas de Ouchterlony (ensayo de interacción antígeno-anticuerpo) y de inmunodifusión radial (RID) (ensayo de cuantificación de antígenos)
Embalaje mejorado El nuevo embalaje respetuoso con el medio ambiente utiliza un 75 % menos de plástico que los frascos originales. Por lo tanto, se emplean menos materias primas y energía en la fabricación, y se generan menos residuos. Además, la boquilla de fácil uso reduce la probabilidad de que se produzcan derrames y contaminación.
Pruebas de rendimiento y calidad El rendimiento de la agarosa UltraPure™ se evalúa para satisfacer las especificaciones establecidas en apariencia, humedad, resistencia del gel, temperatura de gelificación, temperatura de fusión, contenido de sulfato, electroendósmosis y actividad de la DNasa/RNasa.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (aplicación)Electroforesis en gel de ácidos nucleicos, electroforesis de proteínas, transferencia
FormularioPolvo
Compatibilidad del gelGeles de agarosa
Características ecológicasEmbalaje sostenible
Punto de fusiónPunto de fusión estándar
N.º de reacciones2500
Línea de productosUltrapuro
Tipo de productoAgarosa
Cantidad500 g
Condiciones de envíoTemperatura ambiente
Intervalo de separación100 bp a >30 kb
Unit SizeEach
Contenido y almacenamiento
Contenido: 1 bolsa contiene 500 g de agarosa UltraPure™ Almacenamiento: 15°C a 30°C
Preguntas frecuentes
I want to pour my own gels. Which agarose should I use?
Our UltraPure Agarose is standard melting-point agarose designed for routine separation and analysis of DNA and RNA fragments in the 500-23,000 nt range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. We also offer an UltraPure Low Melting Point Agarose, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a low melting temperature of 65°C or less.
How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?
Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
Citations & References (20)
Citations & References
Abstract
Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures.
Authors:Prokopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M,
Journal:Blood
PubMed ID:19369228
'The concept of endothelial progenitor cells (EPCs) has attracted considerable interest in cardiovascular research, but despite a decade of research there are still no specific markers for EPCs and results from clinical trials remain controversial. Using liquid chromatography-tandem mass spectrometry, we analyzed the protein composition of microparticles (MPs) originating from ... More
A phantom for diffusion-weighted MRI (DW-MRI).
Authors:Lavdas I, Behan KC, Papadaki A, McRobbie DW, Aboagye EO,
Journal:J Magn Reson Imaging
PubMed ID:23576443
'PURPOSE: To develop tissue-equivalent diffusivity materials and build a spherical diffusion phantom which mimics the conditions typically found in biological tissues. Also, to assess the reproducibility of ADC measurements from a whole-body diffusion protocol. MATERIALS AND METHODS: Nickel-doped agarose/sucrose gels were manufactured and used to build a spherical diffusion phantom ... More
Determinants of nucleosome organization in primary human cells.
Authors:Valouev A, Johnson SM, Boyd SD, Smith CL, Fire AZ, Sidow A,
Journal:Nature
PubMed ID:21602827
'Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodelling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other functional regions in the genome. Analyses of chromatin landscape have uncovered a variety ... More
Controlling cell position in complex heterotypic 3D microtissues by tissue fusion.
Authors:Rago AP, Dean DM, Morgan JR,
Journal:Biotechnol Bioeng
PubMed ID:19012266
'Tissue fusion and cell sorting are processes fundamental to developmental biology with applications in tissue engineering. We have designed a fusion assay to investigate the factors governing the fusion of microtissues and the cell sorting that occurs after fusion. Normal human fibroblast (NHF) spheroids were self-assembled and cultured for 1, ... More
Host alternation of chikungunya virus increases fitness while restricting population diversity and adaptability to novel selective pressures.
Authors:Coffey LL, Vignuzzi M,
Journal:J Virol
PubMed ID:21047966
'The mechanisms by which RNA arboviruses, including chikungunya virus (CHIKV), evolve and maintain the ability to infect vertebrate and invertebrate hosts are poorly understood. To understand how host specificity shapes arbovirus populations, we studied CHIKV populations passaged alternately between invertebrate and vertebrate cells (invertebrate ? vertebrate) to simulate natural alternation ... More