Ribonuclease H
Ribonuclease H
Citas y referencias (6)
Invitrogen™

Ribonuclease H

La ribonucleasa H (ARNasa H) es una endorribonucleasa que degrada específicamente la cadena de ARN de un híbrido de ARN-ADNMás información
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Número de catálogoCantidad
1802101430 U
18021071120 U
Número de catálogo 18021014
Precio (USD)
372,60
Each
Añadir al carro de la compra
Cantidad:
30 U
Pedido a granel o personalizado
Precio (USD)
372,60
Each
Añadir al carro de la compra
La ribonucleasa H (ARNasa H) es una endorribonucleasa que degrada específicamente la cadena de ARN de un híbrido de ARN-ADN para producir oligorribonucleótidos terminados en fosfato 5´ y ADN de una sola cadena..

Aplicaciones
Extracción de ARNm durante la síntesis de ADNc de segunda cadena. Extracción de secuencias de poli(A) de ARNm en presencia de oligo(dT). Disociación dirigida por oligoribonucleótidos de ARN.

Fuente
Purificada a partir de E. coli que expresa el gen de ARNasa H de E. coli en un plásmido.

Pruebas de rendimiento y calidad
Ribonucleasa, endoexonucleasa inespecífica, endodesoxiribonucleasa 3´ y 5´.

Definición de la unidad
Una unidad hidroliza 1 nmol de ARN en poli(A)poli(dT) marcado como 3H en material soluble en ácido en 20 minutos a 37 °C.

Condiciones de reacción de la unidad
20 mM de Tris-HCl (pH 7,5), 0,1 M de KCl, 10 mM de MgCl2, 0,1 mM de DTT, 5 % (peso/volumen) de sacarosa, 0,5 nmol de poli(A)poli(dT) marcado como 3H y enzima en 50 µl durante 20 min a 37 °C.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de productoRibonuclease H
Cantidad30 U
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Concentración2 U/μl
EnzimaARNasa
Unit SizeEach
Contenido y almacenamiento
Almacenar a -20 °C en un congelador que no forme escarcha

Preguntas frecuentes

Do you offer RNAse H from the SuperScript III First Strand Synthesis System as a stand-alone item?

Yes, we do offer E. coli RNAse H as a stand-alone item: Cat. Nos. 18021014 and 18021071.

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

Citations & References (6)

Citations & References
Abstract
Functions of Transforming Growth Factor-beta Family Type I Receptors and Smad Proteins in the Hypertrophic Maturation and Osteoblastic Differentiation of Chondrocytes.
Authors: Valcourt Ulrich; Gouttenoire Jerome; Moustakas Aristidis; Herbage Daniel; Mallein-Gerin Frederic;
Journal:J Biol Chem
PubMed ID:12082094
'We investigated the effects of bone morphogenetic protein (BMP)-2, a member of the transforming growth factor-beta superfamily, on the regulation of the chondrocyte phenotype, and we identified signaling molecules involved in this regulation. BMP-2 triggers three concomitant responses in mouse primary chondrocytes and chondrocytic MC615 cells. First, BMP-2 stimulates expression ... More
De novo ceramide regulates the alternative splicing of caspase 9 and Bcl-x in A549 lung adenocarcinoma cells. Dependence on protein phosphatase-1.
Authors: Chalfant Charles E; Rathman Kristin; Pinkerman Ryan L; Wood Rachel E; Obeid Lina M; Ogretmen Besim; Hannun Yusuf A;
Journal:J Biol Chem
PubMed ID:11801602
'In a recent study, we showed that ceramide induces the dephosphorylation of SR proteins, a family of protein factors that regulate alternative splicing. In this study, the regulation of the alternative processing of pre-mRNA of both caspase 9 and Bcl-x(L) was examined in response to ceramide. Treatment of A549 lung ... More
The novel WD-repeat protein MORG1 acts as a molecular scaffold for HIF prolyl-hydroxylase 3 (PHD3).
Authors:Hopfer U, Hopfer H, Jablonski K, Stahl RA, Wolf G,
Journal:J Biol Chem
PubMed ID:16407229
'Hypoxia-inducible factor-1 (HIF-1), a transcriptional complex composed of an oxygen-sensitive alpha- and a beta-subunit, plays a pivotal role in cellular adaptation to low oxygen availability. Under normoxia, the alpha-subunit of HIF-1 is hydroxylated by a family of prolyl hydroxylases (PHDs) and consequently targeted for proteasomal degradation. Three different prolyl hydroxylases ... More
High-sensitivity detection of DNA hybridization on microarrays using resonance light scattering.
Authors: Bao Paul; Frutos Anthony G; Greef Charles; Lahiri Joydeep; Muller Uwe; Peterson Todd C; Warden Laurence; Xie Xinying;
Journal:Anal Chem
PubMed ID:11985309
The application of resonance light scattering (RLS) particles for high-sensitivity detection of DNA hybridization on cDNA microarrays is demonstrated. Arrays composed of approximately 2000 human genes ( ... More
mRNA decay is rapidly induced after spore germination of Saccharomyces cerevisiae.
Authors: Brengues Muriel; Pintard Lionel; Lapeyre Bruno;
Journal:J Biol Chem
PubMed ID:12181322
Spores from the yeast Saccharomyces cerevisiae can germinate and resume their vegetative growth when placed in favorable conditions. Biochemical studies on germination have been limited by the difficulty of obtaining a pure population of spores germinating synchronously. Here, we report that spores can be purified and sorted according to their ... More
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