BioNick™ DNA Labeling System
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Citas y referencias (4)
Invitrogen™

BioNick™ DNA Labeling System

El sistema de etiquetado de ADN BioNick™ es ideal para generar sondas de ADN biotiniladas mediante la traslación por muescasMás información
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Número de catálogoCantidad
1824701550 reacciones
Número de catálogo 18247015
Precio (USD)
881,28
Each
Añadir al carro de la compra
Cantidad:
50 reacciones
Precio (USD)
881,28
Each
Añadir al carro de la compra
El sistema de etiquetado de ADN BioNick™ es ideal para generar sondas de ADN biotiniladas mediante la traslación por muescas optimizada para su uso en hibridaciones in situ no radioactivas. Estas sondas también pueden usarse en Southern o Northern blots, elevaciones de placas, hibridaciones de colonias e hibridaciones de transferencias puntuales. Uso del sistema de etiquetado de ADN BioNick™:

• El ADN se etiqueta con biotina-14-dATP, lo que produce tamaños de sonda de 50 a 500 bp
• Una reacción etiqueta 1 µg de ADN molde
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Incluye etiqueta o tinteSí
Método de etiquetadoEtiquetado directo
Línea de productosBioNick
Tipo de productoSistema de etiquetado de ADN
Cantidad50 reacciones
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Método de detecciónA base de biotina
Tipo de producto finalSondas (ADN etiquetado)
Labeling TargetADN (general)
Etiqueta o tinteBiotina
Unit SizeEach
Contenido y almacenamiento
El sistema de etiquetado de ADN BioNick™ incluye una mezcla de 10X dNTP que contiene biotina-14-dATP, una mezcla de enzimas 10X que contiene ADN polimerasa I y DNasa I, ADN de control, tampón de parada y agua destilada. Almacenar a -20 °C. Se garantiza la estabilidad durante 6 meses si se almacena correctamente.

Preguntas frecuentes

What is the difference between the BioNick system and the BioPrime system?

The BioNick Labeling System generates biotinylated DNA probes by nick translation. These probes range from 50 to 500 bases. One reaction labels 1 µg of DNA. The BioPrime Labeling System generates 50-500 ng of biotinylated DNA from 25 ng template DNA by random priming. The probe size ranges from 50 to 700 bases, with a significant portion of the probe being less than 200 bases. See Mackey, J., Rashtchian, A. (1992) FOCUS 14, p.21.

Citations & References (4)

Citations & References
Abstract
Loss of the SKI proto-oncogene in individuals affected with 1p36 deletion syndrome is predicted by strain-dependent defects in Ski-/- mice.
Authors: Colmenares Clemencia; Heilstedt Heidi A; Shaffer Lisa G; Schwartz Stuart; Berk Michael; Murray Jeffrey C; Stavnezer Ed;
Journal:Nat Genet
PubMed ID:11731796
'Experiments involving overexpression of Ski have suggested that this gene is involved in neural tube development and muscle differentiation. In agreement with these findings, Ski-/- mice display a cranial neural tube defect that results in exencephaly and a marked reduction in skeletal muscle mass. Here we show that the penetrance ... More
Cloning and characterization of a family of proteins associated with Mpl.
Authors: Meunier Caroline; Bordereaux Didier; Porteu Francoise; Gisselbrecht Sylvie; Chrétien Stany; Courtois Geneviève;
Journal:J Biol Chem
PubMed ID:11784712
'Thrombopoietin (TPO) controls the formation of megakaryocytes and platelets from hematopoietic stem cells via activation of the c-Mpl receptor and multiple downstream signal transduction pathways. We used two-hybrid screening to identify new proteins that interacted with the cytoplasmic domain of Mpl, and we found a new family of proteins designated ... More
Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.
Authors:Ghanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,
Journal:J Immunol
PubMed ID:15100314
Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More
Structural and functional genomics of the CPT1B gene for muscle-type carnitine palmitoyltransferase I in mammals.
Authors: van der Leij Feike R; Cox Keith B; Jackson Vicky N; Huijkman Nicolette C A; Bartelds Beatrijs; Kuipers Jaap R G; Dijkhuizen Trijnie; Terpstra Peter; Wood Philip A; Zammit Victor A; Price Nigel T;
Journal:J Biol Chem
PubMed ID:12015320
Muscle-type carnitine palmitoyltransferase I (M-CPT I) is a key enzyme in the control of beta-oxidation of long-chain fatty acids in the heart and skeletal muscle. Because knowledge of the mammalian genes encoding M-CPT I may aid in studies of disturbed energy metabolism, we obtained new genomic and cDNA data for ... More