Suero fetal bovino, cualificado, Estados Unidos
Suero fetal bovino, cualificado, Estados Unidos
Citas y referencias (33)
Gibco™

Suero fetal bovino, cualificado, Estados Unidos

Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información
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Número de catálogoCantidadFormato
26140079500 mLFrasco
A316050150 mLOne Shot
A316050210 x 50 mLOne Shot
Our improved Premium Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Número de catálogo 26140079
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Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto

Sueros Gibco: Calidad fiable para un rendimiento uniforme

Categoría de los sueros Gibco: Valor plus (rendimiento)
• Uso para cultivo celular general con líneas de células comunes
• Bajos niveles de endotoxina y hemoglobina
• Nivel de endotoxinas: <10 EU/ml
• Nivel de hemoglobina: <25 mg/dl
• Origen: Estados Unidos

El suero Gibco ofrece
• Certificación ISO 13485, procesado en instalaciones registradas de la FDA
• Etiquetado para uso diagnóstico in vitro (conforme con las pruebas de mayor nivel de esterilidad de la Farmacopea Estadounidense [USP])
• Triple filtrado a 0,1 µm
• El frasco Gibco es más fácil de usar en la campana, reduce el riesgo de contaminación y le ayuda a realizar más coherentemente el cultivo celular.
• El adhesivo extraíble de la etiqueta proporciona una referencia práctica del número de lote y la fecha de caducidad en el cuaderno electrónico de laboratorio
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Concentración de endotoxinas≤ 10 EU/mL
Concentración de hemoglobina≤25 mg/dL
Grado de pureza o calidadCualificado
Condiciones de envíoCongelado
EspecieGanado/bovino
AgeFetal
País de origenEstados Unidos
FormularioLíquido
FormatoFrasco
Tipo de productoSuero fetal bovino
Cantidad500 mL
Serum TreatmentEstándar (esterilizado mediante filtro)
EsterilidadEstéril
Sterilization MethodTriple filtrado, 0,1 μm
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Suero fetal bovino, cualificado, Estados Unidos FAQs

Citations & References (33)

Citations & References
Abstract
A functional role for the B56 alpha-subunit of protein phosphatase 2A in ceramide-mediated regulation of Bcl2 phosphorylation status and function.
Authors: Ruvolo Peter P; Clark Warren; Mumby Marc; Gao Fengqin; May W Stratford;
Journal:J Biol Chem
PubMed ID:11929874
'Recently it has been shown that the potent apoptotic agent ceramide activates a mitochondrial protein phosphatase 2A (PP2A) and promotes dephosphorylation of the anti-apoptotic molecule Bcl2 (Ruvolo, P. P., Deng, X., Ito, T., Carr, B. K., and May, W. S. (1999) J. Biol. Chem. 274, 20296-20300). In cells expressing Bcl2, ... More
Metabolism of 4 beta -hydroxycholesterol in humans.
Authors: Bodin Karl; Andersson Ulla; Rystedt Eva; Ellis Ewa; Norlin Maria; Pikuleva Irina; Eggertsen Gösta; Björkhem Ingemar; Diczfalusy Ulf;
Journal:J Biol Chem
PubMed ID:12077124
'One of the major oxysterols in the human circulation is 4 beta-hydroxycholesterol formed from cholesterol by the drug-metabolizing enzyme cytochrome P450 3A4. Deuterium-labeled 4 beta-hydroxycholesterol was injected into two healthy volunteers, and the apparent half-life was found to be 64 and 60 h, respectively. We have determined earlier the half-lives ... More
Activation of retinoic acid receptor-dependent transcription by all-trans-retinoic acid metabolites and isomers.
Authors: Idres Nadia; Marill Julie; Flexor Maria A; Chabot Guy G;
Journal:J Biol Chem
PubMed ID:12070176
'We have shown that four metabolites of all-trans-retinoic acid (ATRA) (4-oxo-, 4-OH-, 18-OH-, and 5,6-epoxy-RA) can induce maturation of NB4 promyelocytic leukemia cells (Idres, N., Benoit, G., Flexor, M. A., Lanotte, M., and Chabot, G. G. (2001) Cancer Res. 61, 700-705). To better understand the mechanism of action of ATRA ... More
Interaction of human breast fibroblasts with collagen I increases secretion of procathepsin B.
Authors: Koblinski Jennifer E; Dosescu Julie; Sameni Mansoureh; Moin Kamiar; Clark Katherine; Sloane Bonnie F;
Journal:J Biol Chem
PubMed ID:12072442
'Interactions of stromal and tumor cells with the extracellular matrix may regulate expression of proteases including the lysosomal proteases cathepsins B and D. In the present study, we determined whether the expression of these two proteases in human breast fibroblasts was modulated by interactions with the extracellular matrix component, collagen ... More
A ligand-inducible epidermal growth factor receptor/anaplastic lymphoma kinase chimera promotes mitogenesis and transforming properties in 3T3 cells.
Authors: Piccinini Gina; Bacchiocchi Roberta; Serresi Michela; Vivani Caterina; Rossetti Silvia; Gennaretti Claudia; Carbonari Damiano; Fazioli Francesca;
Journal:J Biol Chem
PubMed ID:11919185
'Oncogenic rearrangements of the anaplastic lymphoma kinase (ALK) gene, encoding a receptor type tyrosine kinase, are frequently associated with anaplastic large cell lymphomas. Such rearrangements juxtapose the intracellular domain of ALK to 5''-end sequences belonging to different genes and create transforming fusion proteins. To understand how the oncogenic versions of ... More
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