Estándar de proteínas de NIR preteñido PageRuler™
Estándar de proteínas de NIR preteñido PageRuler™
Thermo Scientific™

Estándar de proteínas de NIR preteñido PageRuler™

La escalera de proteínas NIR preteñida Thermo Scientific PageRuler es una mezcla de 10 proteínas (de 11 a 250 kDa)Más información
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Número de catálogoCantidad
266352 × 250 ml
Número de catálogo 26635
Precio (USD)
-
Cantidad:
2 × 250 ml
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La escalera de proteínas NIR preteñida Thermo Scientific PageRuler es una mezcla de 10 proteínas (de 11 a 250 kDa) teñidas con azul y marcadas con flúor para la visualización fluorescente de infrarrojo cercano y el dimensionamiento de proteínas tras la electroforesis. La escalera de proteínas se suministra en un formato listo para su uso para la carga directa en geles; sin necesidad de calentar, reducir ni añadir un tampón de muestras antes de usarlo.

Compare y visualice todos los demás patrones y escaleras de proteínas ›

Características del producto
• Fluorescente: detección utilizando láser rojo de 670 nm o canales de 700 nm con generadores de imágenes de fluorescencia
• Banda de referencia: la banda de 55 kDa tiene mayor intensidad para facilitar la orientación

Aplicaciones
• Supervisión de la migración de proteínas durante la electroforesis en gel de poliacrilamida-SDS
• Supervisión de la transferencia de proteínas en las membranas tras el Western blot
• Dimensionamiento de proteínas en geles poliacrilamida-SDS fluorescentes NIR y Western blots

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Método de detecciónFluorescencia colorimétrica NIR (700 nm)
Compatibilidad del gelGeles Bolt™ Bis-Tris Plus, Geles de tricina Novex™, geles de Tris-glicina Novex™, geles NuPAGE™ Bis-Tris, geles de Tris-acetato NuPAGE™, geles SDS-PAGE
Peso molecular250, 130, 95, 70, 55, 43, 34, 26, 15, 11 kDa
Línea de productosPageRuler
Tipo de productoMarcadores moleculares de proteínas
Cantidad2 × 250 ml
Listo para cargarSí
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Tipo de tinciónUn color: Azul
Tipo de sistemaWestern Blotting, SDS-PAGE
Number of Markers10
Intervalo de tamañosDe 11 a 250 kDa
Unit SizeEach
Contenido y almacenamiento
Contenido: dos viales de 250 μL cada uno

Tampón de almacenamiento: 62,5 mm de Tris-H3PO4 (pH de 7,5 a 25 °C), 1 mm de EDTA, SDS al 2 %, 10 mm de DTT, 1 mm de NaN3 y glicerol al 33 %

Almacenamiento: Tras su recepción, almacenar a 20 °C

Preguntas frecuentes

Why do Thermo Scientific prestained protein ladders not show the real protein sizes?

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why are the PageRuler and Spectra ladder bands detected with antibodies against eukaryotic proteins?

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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