DMEM, alto contenido en glucosa, sin glutamina, sin rojo fenol
DMEM, alto contenido en glucosa, sin glutamina, sin rojo fenol
Citas y referencias (2)
Gibco™

DMEM, alto contenido en glucosa, sin glutamina, sin rojo fenol

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Más información
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Número de catálogoCantidad
31053028500 mL
3105303610 × 500 mL
Número de catálogo 31053028
Precio (USD)
53,30
Each
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Cantidad:
500 mL
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Precio (USD)
53,30
Each
Añadir al carro de la compra

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM is modified as follows:
WithWithout
• High Glucose• L-glutamine
 • Phenol Red
 • Sodium Pyruvate
 • HEPES


The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de célulasHeLa, 293, Cos-7 y PC-12
Tipo de célulaFibroblastos primarios, neuronas, células gliales, HUVEC y células de músculo liso
Concentración1 X
Concentración de glucosa4500 mg/L
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoDMEM (medio Eagle modificado de Dulbecco)
Cantidad500 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónLibre de material de origen animal
FormularioLíquido
Serum LevelSuplementos de suero estándar
EsterilidadEstéril con filtro
Sterilization MethodEstéril con filtro
Con aditivosAlto contenido en glucosa
Sin aditivosSin glutamina, Sin HEPES, Sin rojo fenol, Sin piruvato sódico
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

Do you offer DMEM, high glucose without phenol red?

We recommend purchasing DMEM, high glucose, no glutamine, no phenol red (Cat. No. 31053028 or 31053036) and supplementing it with 4 mM glutamine and 1 mM sodium pyruvate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all DMEM, alto contenido en glucosa, sin glutamina, sin rojo fenol FAQs

Citations & References (2)

Citations & References
Abstract
Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain.
Authors:Gauthier SA, Sahoo S, Jung SS, Levy E
Journal:Methods Mol Biol
PubMed ID:22528096
'The use of murine cerebrovascular endothelial and smooth muscle cells has not been widely employed as a cell culture model for the investigation of cellular mechanisms involved in cerebral amyloid angiopathy (CAA). Difficulties in isolation and propagation of murine cerebrovascular cells and insufficient yields for molecular and cell culture studies ... More
Potentially novel candidate biomarkers for head and neck squamous cell carcinoma identified using an integrated cell line-based discovery strategy.
Authors:Sepiashvili L, Hui A, Ignatchenko V, Shi W, Su S, Xu W, Huang SH, O'Sullivan B, Waldron J, Irish JC, Perez-Ordonez B, Liu FF, Kislinger T
Journal:Mol Cell Proteomics
PubMed ID:22918226
Head and neck squamous cell carcinomas (HNSCC) can arise from the oral cavity, oropharynx, larynx or hypopharynx, and is the sixth leading cancer by incidence worldwide. The 5-year survival rate of HNSCC patients remains static at 40-60%. Hence, biomarkers which can improve detection of HNSCC or early recurrences should improve ... More