DMEM, alto contenido en glucosa, sin glutamina, sin rojo fenol

Citas y referencias (2)

Gibco™

DMEM, alto contenido en glucosa, sin glutamina, sin rojo fenol

Name: DMEM, alto contenido en glucosa, sin glutamina, sin rojo fenol
SKU: 31053036

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Más información

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Número de catálogo	Cantidad
31053036	10 × 500 mL
31053028	500 mL

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Número de catálogo 31053036

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Cantidad:

10 × 500 mL

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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• L-glutamine
	• Phenol Red
	• Sodium Pyruvate
	• HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Línea de célulasHeLa, 293, Cos-7, and PC-12

Tipo de célulaPrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

Concentración1 X

Calidad de fabricacióncGMP-compliant under the ISO 13485 standard

Línea de productosGibco

Tipo de productoDMEM (Dulbecco's Modified Eagle Medium)

Cantidad10 × 500 mL

Duración de almacenamiento12 Months From Date of Manufacture

Condiciones de envíoTemperatura ambiente

ClasificaciónAnimal Origin-free

FormularioLíquido

Serum LevelSuplementos de suero estándar

EsterilidadEstéril con filtro

Sterilization MethodSterile-filtered

Con aditivosHigh Glucose

Sin aditivosNo Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

Do you offer DMEM, high glucose without phenol red?

We recommend purchasing DMEM, high glucose, no glutamine, no phenol red (Cat. No. 31053028 or 31053036) and supplementing it with 4 mM glutamine and 1 mM sodium pyruvate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM, alto contenido en glucosa, sin glutamina, sin rojo fenol FAQs

Citations & References (2)

Citations & References

Abstract

Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain.

Authors:Gauthier SA, Sahoo S, Jung SS, Levy E

Journal:Methods Mol Biol

PubMed ID:22528096

'The use of murine cerebrovascular endothelial and smooth muscle cells has not been widely employed as a cell culture model for the investigation of cellular mechanisms involved in cerebral amyloid angiopathy (CAA). Difficulties in isolation and propagation of murine cerebrovascular cells and insufficient yields for molecular and cell culture studies ... More

Potentially novel candidate biomarkers for head and neck squamous cell carcinoma identified using an integrated cell line-based discovery strategy.

Authors:Sepiashvili L, Hui A, Ignatchenko V, Shi W, Su S, Xu W, Huang SH, O'Sullivan B, Waldron J, Irish JC, Perez-Ordonez B, Liu FF, Kislinger T

Journal:Mol Cell Proteomics

PubMed ID:22918226

Head and neck squamous cell carcinomas (HNSCC) can arise from the oral cavity, oropharynx, larynx or hypopharynx, and is the sixth leading cancer by incidence worldwide. The 5-year survival rate of HNSCC patients remains static at 40-60%. Hence, biomarkers which can improve detection of HNSCC or early recurrences should improve ... More