Keratinocyte-SFM Supplement: Human Recombinant Epidermal Growth Factor (EGF 1-53) & Bovine Pituitary Extract (BPE)

Gibco™

Keratinocyte-SFM Supplement: Human Recombinant Epidermal Growth Factor (EGF 1-53) & Bovine Pituitary Extract (BPE)

Factor de crecimiento epidérmico recombinante humano (EGF 1-53) y extracto pituitario bovino (BPE).Más información

Número de catálogo	Cantidad
37000015	1 mL

Número de catálogo 37000015

Precio (USD)

133,25

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Cantidad:

Precio (USD)

133,25

Añadir al carro de la compra

Factor de crecimiento epidérmico recombinante humano (EGF 1-53) y extracto pituitario bovino (BPE).

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaCélulas de queratinocitos, células epiteliales

Tipo de cultivoCultivo de células de mamíferos

Cantidad1 mL

Nivel de sueroSin suero

Duración de almacenamiento18 meses

Condiciones de envíoHielo seco

EspecieHumano

ClasificaciónSin suero

Tipo de productoSuplemento de medio sin suero (SFM) de queratinocitos

EsterilidadEstéril

Sterilization MethodEstériles, con filtro

Con aditivosPhenol Red

Unit SizeEach

Contenido y almacenamiento

Almacenar en congelador (de – 5 a – 30 °C).

Preguntas frecuentes

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.