TaqMan™ RNase P Instrument Verification Plate, 96-well
Product Image
Applied Biosystems™

TaqMan™ RNase P Instrument Verification Plate, 96-well

La placa de verificación de instrumentos ARNasa P TaqMan® se utiliza para verificar el rendimiento del sistema de PCR enMás información
Have Questions?
Número de catálogoCantidad
43109821 placa de 96 pocillos
Número de catálogo 4310982
Precio (USD)
1.013,88
Each
Añadir al carro de la compra
Cantidad:
1 placa de 96 pocillos
Precio (USD)
1.013,88
Each
Añadir al carro de la compra
La placa de verificación de instrumentos ARNasa P TaqMan® se utiliza para verificar el rendimiento del sistema de PCR en tiempo real de 96 pocillos Applied Biosystems® 7900HT y de los sistemas de detección de secuencias ABI PRISM® 7000 y 7700.La placa de verificación de instrumentos de ARNasa P TaqMan® es una placa de PCR de 96 pocillos que trae precargados los reactivos necesarios para detectar y cuantificar copias genómicas del gen humano ARNasa P, un gen de una sola copia que codifica la fracción de ARN de la enzima ARNasa P.Cada pocillo contiene una mezcla de reacción precargada (1X mezcla maestra universal de PCR TaqMan®, primers de ARNasa P y sonda de etiquetado con tinte FAM™) y la plantilla.La placa de verificación de instrumentos ARNasa P TaqMan® cumple los mismos requisitos que el sistema de PCR en tiempo real Applied Biosystems® 7900HT de 96 pocillos y las instalaciones de los sistemas de detección de secuencias ABI PRISM® 7000 y 7700.Para aprobar la instalación, los instrumentos deben demostrar la capacidad de distinguir entre 5000 y 10.000 equivalentes genómicos con un nivel de confianza del 99,7 % para un posterior procesamiento de muestras en un solo pocillo.Para garantizar resultados exactos, Applied Biosystems® le recomienda verificar el rendimiento de sus instrumentos cada seis meses.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tinte calibradoFAM
Tipo de calibraciónCalibración ARNasa P, placa de verificación de instrumentos
Método de detecciónFluorescente
Para utilizar con (aplicación)PCR en tiempo real (PCRq)
Para utilizar con (equipo)Sistema 7000, sistema 7700, sistema 7900HT
Formato96-well
Línea de productosTaqMan
Cantidad1 placa de 96 pocillos
Condiciones de envíoHielo seco
Tipo de productoPlaca de verificación del instrumento
Unit SizeEach
Contenido y almacenamiento
Contiene una placa de reacción de 96 pocillos precargada y sellada con el ensayo de 5’ nucleasa utilizando reactivos TaqMan™ ARNasa P, incluidos cebadores y sondas. Almacenar a entre -15 y 25 °C. Proteja el producto de la luz.

Preguntas frecuentes

How can I convert a file from AQ to RQ, or from RQ to AQ file with the 7900HT Fast Real-Time PCR System?

SDS v2.4 and above allow you to convert your AQ files to RQ and vice versa.
To do so, simply go to: Tools then AQ RQ Converter
-Choose from the dropdown which direction you want to go (AQ to RQ, or RQ to AQ).
-Next click on ‘Add' and browse to the plate file location in the new window. and click ‘Open'.
-Click on ‘Convert'. A new file will be created in the same location as the original, but with _AQ or _RQ at the end of the filename.

If you do not have v2.4, you can upgrade your software here (http://www.thermofisher.com/us/en/home/technical-resources/software-downloads/applied-biosystems-7900ht-fast-real-timespcr-system.html).

One benefit of this conversion feature is that it allows you to convert 7900 files to the correct type to do analysis for relative quantification study, for example, if a plate is originally run as AQ instead of RQ, or if you would like to generate a standard curve quantification in the AQ if a plate was originally run as RQ.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why are there no RQ values for all the samples in my study in the 7900HT Fast Real-Time PCR System software?

The 7900HT Fast Real-Time PCR System software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have Ct values for all the wells, but the software will not calculate RQ values. If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/dataassist-software.html?icid=fr-realtimesoftware-5) instead, or use the Thermo Fisher Cloud (https://www.thermofisher.com/us/en/home/cloud.html). This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7900HT software as a *.txt or *.csv file, and use that to create a new study in the DataAssist software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why do I not see any Ct or RQ values from my ddCT experiment in the 7900HT Fast Real-Time PCR System software?

In the 7900HT Fast Real-Time PCR System software, v2.3 or later, CT or RQ values are only provided in the RQ Manager software. RQ Manager is installed on the computer along with SDS v2.x.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What can I use for data analysis on the 7900HT Fast Real-Time PCR System?

For genotyping data, we recommend TaqMan Genotyper Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/taqman-genotyper-software.html?icid=fr-software-1).
For relative quantitation, we recommend ExpressionSuite Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/expressionsuite-software.html). You may also use the Thermo Fisher Cloud for either application. https://www.thermofisher.com/us/en/home/cloud.html

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What dyes can I use on the 7900HT Fast Real-Time PCR System?

The Applied Biosystems 7900HT Fast Real-Time PCR Systems use the following dye sets for calibration: FAM, SYBR, VIC, ROX, NED, TAMRA, JOE, TET dyes. Custom dyes can also be used that read between 500 nm and 660 nm, although you will have to calibrate the system first for any new dye.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.