Ensayo de genotipado SNP TaqMan™, humano
Ensayo de genotipado SNP TaqMan™, humano
Citas y referencias (1421)
Applied Biosystems™

Ensayo de genotipado SNP TaqMan™, humano

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Los ensayos de identificación de genotipos de SNP Applied Biosystems TaqMan utilizan la composición química de la 5´-nucleasa TaqMan paraMás información
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Número de catálogoCantidad
43513742400 reacciones (L; 80X)
43513761000 reacciones (M, 40X)
4351379300 reacciones (pequeñas, 40X)
Número de catálogo 4351374
Precio (USD)
-
Cantidad:
2400 reacciones (L; 80X)
Los ensayos de identificación de genotipos de SNP Applied Biosystems TaqMan utilizan la composición química de la 5´-nucleasa TaqMan para amplificar y detectar polimorfismos específicos en muestras de ADN genómico purificado.Cada ensayo permite el genotipado de individuos para un polimorfismo de nucleótido único (SNP) y consta de dos primers específicos de secuencia y dos sondas de ligante de ranura menor TaqMan (MGB) con supresores no fluorescentes (NFQ).Una sonda está etiquetada con colorante VIC para detectar la secuencia Allele 1; la segunda sonda está etiquetada con colorante FAM para detectar la secuencia Allele 2.

Nuestros ensayos humanos de genotipado TaqMan SNP prediseñados son una colección de millones de ensayos humanos para todo el genoma, incluidos 1000 SNP de genomas, SNP de HapMap y SNP de codificación.

Ventajas:
•Probado: la química TaqMan estándar de oro y los diseños de ensayo robustos brindan resultados precisos, reproducibles y confiables
•Sencillo: el conveniente formato de un solo tubo y el flujo de trabajo simple brindan un camino fácil hacia resultados confiables; no se requiere optimización
•Relevante: la extensa colección de ensayos humanos prediseñados ofrece acceso directo al contenido que es relevante para su investigación.
•Probado: todos los ensayos de genotipado de SNP humanos se prueban funcionalmente para garantizar la discriminación alélica

Tiempo aproximado de envío
4–6 días en Norteamérica y 6–10 días en Europa

Los ensayos de genotipado TaqMan SNP requieren solo tres componentes de reacción para PCR: ADN genómico purificado (1–20 ng), la solución de ensayo y la mezcla maestra de genotipado TaqMan (u otra mezcla maestra compatible) (se vende por separado).

Todos los diseños de ensayos son el producto de nuestra cartera de bioinformática, optimizada a lo largo de más de una década mediante el aprovechamiento de los datos de rendimiento de ensayo y fabricación.Los ensayos TaqMan se han citado en más de 40 000 publicaciones y están respaldados por más de 350 patentes.

Todos nuestros ensayos TaqMan prediseñados están cubiertos por la garantía qPCR de los ensayos TaqMan.*

Mezcla maestra recomendada (se vende por separado):Mezcla maestra para genotipado TaqMan

*Se aplican los términos y condiciones.Para obtener más información, visite www.thermofisher.com/taqmanguarantee.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Modificación del cebador 3'Ninguno
Modificación del cebador 5'Ninguno
DescripciónLa sonda de MGB VIC™ detecta el alelo 1; la sonda FAM-MGB detecta el alelo 2
Para utilizar con (equipo)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ Absolute Q Digital PCR System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals.
Características ecológicasEmbalaje sostenible
Modificación de sonda internaVIC™ (5'), FAM (5'), MGB (unión al surco menor) (3')
N.º de reacciones12.000 reacciones
Línea de productosTaqMan
Método de purificaciónExtracción en fase sólida (SPE)
Grado de pureza o calidadSin ARNasa, sin ADNasa
Cantidad2400 reacciones (L; 80X)
Tipo de muestraHumano
Condiciones de envíoTemperatura ambiente
EspecieHumano
ObjetivoHay más de 4,5 millones de ensayos genómicos en humanos disponibles
Concentración80X
Para utilizar con (aplicación)Genotipado
FormularioCongelado
Etiqueta o tinteFAM, VIC
Unit SizeEach
Contenido y almacenamiento
1 tubo que contiene una mezcla de 40x (tamaños S y M) u 80x (tamaño L) de ensayo preformulado (2 sondas y 2 cebadores).

Almacenar a entre -15 y -25 °C.

Preguntas frecuentes

How do I set up a reference panel in the TaqMan Genotyper Software?

A reference panel is helpful in large studies to mark your reference samples. Please follow the directions here on how to set up a reference panel.

How do I enter the polymorphism sequence information (i.e., A, C, G, T) for my assays, and where is this info displayed in the TaqMan Genotyper Software?

The polymorphism sequence info can be entered into the software through Setup >Assays. You can import an assay information file (AIF) that contains this info for your assays (AIFs are shipped with assay orders), or manually enter this info for each assay using the edit assay feature. The polymorphism sequence info will be displayed in the assays table under allele1 base and allele2 base, in the results table in the calls column, in the cluster plot display in the x-axis and y-axis titles, and in the export files as genotypes. If no sequence information is entered for an assay, the default display for genotype calls will use the dye names, such as VIC/VIC, VIC/FAM or FAM/FAM dyes.

What is the bookmarking feature in the TaqMan Genotyper Software, and how would I use it?

Bookmarking is a unique feature in TaqMan Genotyper Software that allows you to tag a data point or well while reviewing results in a Study. For example, in reviewing a cluster plot for an assay, a data point is observed to be somewhat between clusters. You can set a bookmark for this data point to denote this well for further investigation. The bookmark persists between the Results workspace and Quality Control workspace, so you can easily identify the data point in a cluster plot, experiment plate view, or on the samples tab. Bookmarks are cleared upon exit from a Study or exit from the application.

I am getting the message: "An error has occurred. See the log file C:\ProgramFiles\Applied Biosystems\TaqMan Genotyper\config\eclipse\1363113099385.log." How can I fix this?

1.Go to the Start button, then Programs, then TaqMan Genotyper Software
2.Right-click on the program and choose “Run as Administrator”
3.If that does not work, go back to the same menu and choose “Properties”
4.Choose the “Compatibility” tab, and check “Run this program as administrator”
5.Click “Apply”
6.You may+C69 have to restart the computer for the settings to apply

Can I delete an assay or sample from my qPCR study?

An assay or sample may be deleted from a study only if there is no data or wells associated with it. Upon import of an experiment, the software collects all the assays and samples from the plate and lists them in the Setup > Assays or Setup > Samples workspaces. The assays and samples are stored in these workspaces as a library, and remain there even if you delete the experiment from the Study. Deleting the experiment will remove any data (wells) associated with the assays or samples, but not the assays or samples from the library. The assays and samples must then be deleted from these workspaces to remove them from the Study.

View all Ensayo de genotipado SNP TaqMan™, humano FAQs

Citations & References (1421)

Citations & References
Abstract
Determination, mechanism and monitoring of knockdown resistance in permethrin-resistant human head lice, Pediculus humanus capitis
Authors:Clark, JM
Journal:JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
PubMed ID:
Permethrin resistance has been reported worldwide and clinical failures to commercial pediculicides containing permethrin have likewise occurred. Permethrin resistance in head lice populations from the U.S. is widespread but is not yet uniform and the level of resistance is relatively low (∼4-8 fold). Permethrin-resistant lice are cross-resistant to pyrethrins, PBO-synergized ... More
Geographical mapping of a multifocal thyroid tumour using genetic alteration analysis & miRNA profiling
Authors:Aherne, ST; Smyth, PC; Flavin, RJ; Russell, SM; Denning, KM; Li, JH; Guenther, SM; O'Leary, JJ; Sheils, OM
Journal:Molecular Cancer
PubMed ID:
Background: Papillary thyroid carcinoma (PTC) frequently presents as multiple tumour-foci within a single thyroid gland or pluriform, with synchronous tumours comprising different histological variants, raising questions regarding its clonality. Among the genetic aberrations described in PTC, the BRAF V600E mutation and ret/PTC activation occur most commonly. Several studies have investigated ... More
Risk haplotype analysis for bovine paratuberculosis
Authors:Pinedo, PJ; Wang, CG; Li, Y; Rae, DO; Wu, RL
Journal:MAMMALIAN GENOME
PubMed ID:
Paratuberculosis (Johne's disease), caused by Mycobacterium avium subsp. paratuberculosis, is an important disease for bovines, although its genetic basis is poorly understood. In this study, three candidate genes were typed to study the associations between single nucleotide polymorphisms (SNPs) and paratuberculosis susceptibility (measured in a 1 or 0 form) at ... More
Risk haplotype analysis for bovine paratuberculosis
Authors:Pinedo, PJ; Wang, CG; Li, Y; Rae, DO; Wu, RL
Journal:JOURNAL OF NEURAL TRANSMISSION
PubMed ID:
Paratuberculosis (Johne's disease), caused by Mycobacterium avium subsp. paratuberculosis, is an important disease for bovines, although its genetic basis is poorly understood. In this study, three candidate genes were typed to study the associations between single nucleotide polymorphisms (SNPs) and paratuberculosis susceptibility (measured in a 1 or 0 form) at ... More
Association of Polymorphisms in Genes of the Homologous Recombination DNA Repair Pathway and Thyroid Cancer Risk
Authors:Bastos, HN; Antao, MR; Silva, SN; Azevedo, AP; Manita, I; Teixeira, V; Pina, JE; Gil, OM; Ferreira, TC; Limbert, E; Rueff, J; Gaspar, JF
Journal:THYROID
PubMed ID:
Background: Ionizing radiation exposure has been pointed out as a risk factor for thyroid cancer. The double-strand breaks induced by this carcinogen are usually repaired by homologous recombination repair pathway, a pathway that includes several polymorphic genes. Since there is a scarcity of data about the involvement of these gene ... More
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