StepOne™ Real-Time PCR System Spectral Calibration Kit (48 well)

Este kit fácil de usar establece los espectros de colorante puro y los valores de varios componentes para los colorantesMás información

Número de catálogo	Cantidad
4371433	1 kit

Número de catálogo 4371433

Precio (USD)

913,88

Añadir al carro de la compra

Cantidad:

1 kit

Precio (USD)

913,88

Añadir al carro de la compra

Este kit fácil de usar establece los espectros de colorante puro y los valores de varios componentes para los colorantes FAM™/SYBR™ Green I, VIC™/JOE™ y ROX™ en el sistema PCR en tiempo real StepOne™.

Las características del kit de calibración espectral del sistema de PCR en tiempo real StepOne™ incluyen:

• Se proporcionan placas de reacción óptica de 48 pocillos precargadas con colorantes (FAM™/SYBR™ Green I, VIC™/JOE™y ROX™) para una calibración rápida y sencilla del sistema de PCR en tiempo real StepOne™
• Fácil de utilizar, proporciona discriminación precisa de la señal de fluorescencia de colorantes individuales en la mezcla de reacción
• Incluye el patrón de colorante Referencia pasiva I para la normalización de la señal en todas las reacciones en tiempo real y de punto final

El kit de calibración espectral del sistema de PCR en tiempo real StepOne™ de Applied Biosystems™ contiene una placa de fondo y tres placas de reacción óptica rápida precargadas de 48 pocillos, con cinco patrones de colorante.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tinte calibradoFAM, JOE, ROX, VIC, SYBR™ Green

Tipo de calibraciónCalibración espectral

Método de detecciónFluorescente

Para utilizar con (aplicación)PCR en tiempo real (PCRq)

Para utilizar con (equipo)StepOne™, modo Fast, StepOne™, modo estándar

Línea de productosStepOne

Cantidad1 kit

Condiciones de envíoHielo seco

Tipo de productoKit de calibración espectral

Unit SizeEach

Contenido y almacenamiento

Contiene tres placas de reacción de 48 pocillos: una placa de fondo y dos que incluyen los patrones de colorante (placas I y II). La placa I contiene FAM, JOE y ROX; la placa II contiene SYBR™ Green I y VIC.

Almacenar a entre -15 y 25 °C. Proteger de la luz.

Fecha de caducidad: 12 meses a partir de la fecha de entrega.

Preguntas frecuentes

What does "Error - Cannot calculate Pure Dye Matrix" mean?

This issue can occur if the run is interrupted. To prevent this from happening in the future, avoid ending the PCR with an infinite hold stage or interrupting the run. Please send the file to techsupport@thermofisher.com so that our software group can attempt to recover the data.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Fatal Error Number 0x8158" mean?

This is generally caused by incorrect placement of tube strips, resulting in a sample block that can't properly be lowered. We recommend contacting Instrument Services to have an engineer walk you through a manual drawer-lowering process during instrument power-up. To prevent this from happening in the future, place tubes toward the middle of the block or use a placeholder strip on the opposing side to counterbalance block pressure.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Java.lang.IllegalArgumentException: One of the raw spectra is null" mean?

This issue can occur if the “Start Run” button is clicked more than once or if illegal characters are used in the experiment/file name. You can recover the data yourself by overriding the calibration file with one that represents the calibration status of the instrument when you ran that particular file. Use the toolbar at the top to navigate: Analysis then Override Calibration then Use Calibration From Another File.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Cannot open the file because file contents are not recognized" mean?

This issue can occur if duplicate samples are added to the experiment, if illegal characters are used in the experiment/file name, or if the file extension was changed to .eds from a different extension.
Please send the file to techsupport@thermofisher.com to attempt recovery.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Analysis failed due to GExSession doesn't exist in context" mean?

This error means that the data collection was not turned on as appropriate. If this is inadvertently turned off, no data will be collected. Thus, the data are not retrievable in such a file.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all StepOne™ Real-Time PCR System Spectral Calibration Kit (48 well) FAQs