TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)
TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)
Citas y referencias (52)
Applied Biosystems™

TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you toMás información
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Número de catálogoN.º de reacciones
439112840 reacciones
Número de catálogo 4391128
Precio (USD)
1.293,36
Each
Añadir al carro de la compra
N.º de reacciones:
40 reacciones
Precio (USD)
1.293,36
Each
Añadir al carro de la compra
TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

TaqMan™ PreAmp Master Mix lets you:

• Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays
• Analyze up to 100 gene expression targets with minimal hands-on time

Preserves Equilibrium of Targets Without Preamplification Bias
In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.

Easy Workflow
With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Para utilizar con (equipo)Sistema 7500 Fast, Sistema 7500, Sistema 7900HT, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, Sistema ViiA™ 7
Inicio en calienteInicio en caliente integrado
N.º de reacciones40 reacciones
Colorante de referencia pasivaSin ROX
PolimerasaADN polimerasa AmpliTaq Gold
Línea de productosTaqMan
Tipo de productoMezcla maestra PreAmp
Cantidad40 reactions
Tipo de muestraADNc
Suficiente para40 reacciones
Volumen1 mL
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Preamplificación
GC-Rich PCR PerformanceAlto
Método de PCRqPCR
Unit SizeEach
Contenido y almacenamiento
Contiene mezcla maestra de preamplificador TaqMan™ 2X (1 ml). Almacenar a 28 °C

La vida útil mínima garantizada es de 60 días (fecha de caducidad exacta impresa en el producto y en el certificado de análisis).

Preguntas frecuentes

Can I use preamplification with the TaqMan SNP Genotyping Assays?

There is a preamplification protocol included with our TaqMan Sample-to-SNP Kit. This is designed to work with a lysate sample.

I want to purchase TaqMan Array Human MicroRNA A Cards v2.0 (Cat. No. 4398965). What other reagents do I need to purchase in addition to these cards?

In order to perform reverse transcription on these cards, you will need the following:

  • TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)
  • Megaplex RT Primers pools (for human Type A cards Cat. No. 4399966, for Type B cards Cat. No. 4444281)
You can proceed with or without a preamplification step which depends on the total RNA amount. Total RNA amount of 1‐1000 ng supports reverse transcription reaction with preamplification, whereas 350‐1000 ng of total RNA supports a reaction without preamplification. In case you are performing preamplification you would also need the TaqMan PreAmp Master Mix (Cat. No. 4391128) and the Megaplex PreAmp Primers, Human Pool A v2.1 (Cat. No. 4399233). You will also need the Master Mix for the qPCR reaction. It is not included in the card kit. The recommended Master Mix is: TaqMan Fast Advanced Master Mix – run mode Fast (Cat. No. 4444556) For further information, please see the following link.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

How does the SuperScript IV Single Cell/Low Input cDNA PreAmp Kit differ from TaqMan PreAmp Master Mix?

TaqMan PreAmp Master Mix is designed for targeted preamplification of up to 100 targets from small amounts of cDNA, while SuperScript IV Single Cell/Low Input cDNA PreAmp Kit allows global preamplification of full-length cDNA from single cells or low input RNA. It provides reagents for cell lysis, reverse transcription and preamplification steps.

How do I reorder the TaqMan PreAmp Master Mix, Cat No. 4384266? It does not match the Cat. No. when I search online.

This is an internal part number for the TaqMan PreAmp Master Mix. To order, please use Cat No. 4391128 for the 40 reaction size or Cat. No. 4488593 for the 200 reaction size. This is the same product.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Citations & References (52)

Citations & References
Abstract
Target genes of neuron-restrictive silencer factor are abnormally up-regulated in human myotilinopathy.
Authors:Barrachina M, Moreno J, Juves S, Moreno D, Olive M, Ferrer I
Journal:Am J Pathol
PubMed ID:17823282
Myotilinopathy is a subgroup of myofibrillar myopathies caused by mutations in the myotilin gene in which there is aggregation of abnormal cytoskeletal proteins and ubiquitin. We report here on the accumulation of neuron-related proteins such as ubiquitin carboxy-terminal hydrolase L1 (UCHL1), synaptosomal-associated protein 25, synaptophysin, and alpha-internexin in aberrant protein ... More
Differential expression of microRNA-675, microRNA-139-3p and microRNA-335 in benign and malignant adrenocortical tumours.
Authors:Schmitz KJ, Helwig J, Bertram S, Sheu SY, Suttorp AC, Seggewiss J, Willscher E, Walz MK, Worm K, Schmid KW
Journal:J Clin Pathol
PubMed ID:21471143
'Background For the clinical management of adrenocortical neoplasms it is crucial to correctly distinguish between benign and malignant tumours. Even histomorphologically based scoring systems do not allow precise separation in single lesions, thus novel parameters are desired which offer a more accurate differentiation. The tremendous potential of microRNAs (miRNAs) as ... More
Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu.
Authors:Reed-Geaghan EG, Reed QW, Cramer PE, Landreth GE
Journal:J Neurosci
PubMed ID:21084593
'Alzheimer''s disease (AD) is characterized by the deposition of beta-amyloid (Abeta)-containing plaques within the brain that is accompanied by a robust microglial-mediated inflammatory response. This inflammatory response is reliant upon engagement of innate immune signaling pathways involving the toll-like receptors (TLRs). Studies assessing the roles of TLRs in AD pathogenesis ... More
Anti-epithelial cell adhesion molecule antibodies and the detection of circulating normal-like breast tumor cells.
Authors:Sieuwerts AM, Kraan J, Bolt J, van der Spoel P, Elstrodt F, Schutte M, Martens JW, Gratama JW, Sleijfer S, Foekens JA
Journal:J Natl Cancer Inst
PubMed ID:19116383
'Identification of specific subtypes of circulating tumor cells in peripheral blood of cancer patients can provide information about the biology of metastasis and improve patient management. However, to be effective, the method used to identify circulating tumor cells must detect all tumor cell types. We investigated whether the five ... More
Altered GABAA receptor-mediated synaptic transmission disrupts the firing of gonadotropin-releasing hormone neurons in male mice under conditions that mimic steroid abuse.
Authors:Penatti CA, Davis MC, Porter DM, Henderson LP
Journal:J Neurosci
PubMed ID:20463213
'Gonadotropin-releasing hormone (GnRH) neurons are the central regulators of reproduction. GABAergic transmission plays a critical role in pubertal activation of pulsatile GnRH secretion. Self-administration of excessive doses of anabolic androgenic steroids (AAS) disrupts reproductive function and may have critical repercussions for pubertal onset in adolescent users. Here, we demonstrate that ... More
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