GeneChip™ Rat Genome 230 2.0 Assay
Applied Biosystems™

GeneChip™ Rat Genome 230 2.0 Assay

The GeneChip™ Rat Genome 230 2.0 Assay is a powerful tool for toxicology, neurobiology, and other applications using rat asMás información
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Número de catálogoNúmero de arrays
90249210 matrices
90249330 matrices
Número de catálogo 902492
Precio (USD)
-
Número de arrays:
10 matrices
The GeneChip™ Rat Genome 230 2.0 Assay is a powerful tool for toxicology, neurobiology, and other applications using rat as a model organism.

• Provides comprehensive coverage of the transcribed rat genome on a single array
• Comprised of more than 31,000 probe sets, analyzing over 30,000 transcripts and variants from over 28,000 well-substantiated rat genes
• Sophisticated bioinformatics tools available through the NetAffx™ Analysis Center to rapidly obtain biologically meaningful results
• Publicly available drafts of the rat genome and leading public rat databases were used to refine sequences and provide a higher quality of data output

Assay Profile
All probe sets represented on the GeneChip Rat Expression Set 230 are included on the GeneChip Rat Genome 230 2.0 Assay. Sequences used in the design of the GeneChip Rat Genome 230 2.0 Assay were selected from GenBank™, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 99, June 2002) and then refined by analysis and comparison with the publicly available draft assembly of the rat genome from the Baylor College of Medicine Human Genome Sequencing Center (June 2002).

The GeneChip Rat Genome 230 2.0 Assay includes representation of the RefSeq database sequences and probe sets related to sequences and refined EST clusters previously represented on the GeneChip Rat Genome U34 Set.

Oligonucleotide probes complementary to each corresponding sequence are synthesized in situ on the arrays. Eleven pairs of oligonucleotide probes are used to measure the level of transcription of each sequence represented on the GeneChip Rat Genome 230 2.0 Assay.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Para utilizar con (aplicación)Microarray Analysis
Línea de productosGeneChip
Cantidad10 reactions
matriz, red, conjuntoTranscriptome Profiling
FormatoArray Cartridge
Número de arrays10 matrices
EspecieRat
Unit SizeEach

Preguntas frecuentes

Is it possible to compare different array types (e.g., Rat 230A and Rat 230 2.0) in GeneChip Command Console (GCC)?

GeneChip Command Console cannot generate a comparison analysis between different array types.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How long does the scan of a Rat 230 2.0 Array take?

Scanning a single Rat 230 2.0 array takes approximately 9 minutes. This is about 25% less time than is required to scan the Rat 230A and Rat 230B arrays combined.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How much memory is needed to store the data from a single Rat 230 2.0 Array?

A single Rat 230 2.0 array generates approximately 92 MB of data.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Where can I find the high-resolution scanning patch for the Rat 230 2.0 Array?

Please contact Technical Support to obtain the high-resolutiion scanning patch.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Do I have to apply the high-resolution scanning patch for the Rat 230 2.0 Array?

Below are a list of circumstances and clarification on if the high-resolution scanning patch needs to be applied. Keep in mind that the high-resolution scanning patch must only be applied on top of GCOS, not AGCC software.

a. GeneChip Scanner 3000 (serial numbers 502xxxxx): The instrument service engineer has applied the high-resolution scanning patch to the computer that is controlling the GeneChip Scanner 3000 at installation, but not to the workstations or servers (see c and d below).

b. GeneChip Scanner 3000 (serial numbers 501xxxxx): The instrument service engineer will apply the high-resolution scanning patch to the computer that is controlling the GeneChip Scanner 3000 at the time of the upgrade, but not to the workstations or servers (see c and d below).

c. Analysis workstations and clients: You must apply the high-resolution scanning patch. d. Server upgrade: Your database administrator or an Affymetrix Software Support Engineer needs to apply the high-resolution scanning patch in coordination with the upgrade of server to GCOS.

e. Installation of library files post scanner installation or upgrade: The high-resolution scanning patch must be reapplied following the installation of library files for arrays that precede the Rat 230 2.0 Array. The Rat 230 2.0 array and library files are high-resolution-scanning ready. The error message "cannot read the parameters for probe array type from the database" appears when one tries to scan an array on a GeneChip Scanner 3000 high-resolution scanner but the high-resolution scanning patch has not been applied

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.