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Citas y referencias (18)
Invitrogen™
ATTO-TAG™ FQ Derivatization Reagent (FQ; 3-(2-Furoyl)quinoline-2-Carboxaldehyde)
El reactivo ATTO-TAG FQ permite la detección ultrasensible de aminas primarias. Este reactivo está disponible como producto independiente o comoMás información
| Número de catálogo | Cantidad |
|---|---|
| A10192 | 10 mg |
Número de catálogo A10192
Precio (USD)
-
Cantidad:
10 mg
El reactivo ATTO-TAG FQ permite la detección ultrasensible de aminas primarias. Este reactivo está disponible como producto independiente o como componente de un kit de derivatización de aminas (A-2334). Debido a que ATTO-TAG FQ no es fluorescente en el estado no reaccionado y reacciona específicamente con aminas para formar conjugados altamente fluorescentes, es especialmente adecuado para la derivatización de péptidos, aminoácidos, azúcares aminados y otras moléculas que contienen aminas para la detección por electroforesis capilar con fluorescencia inducida por láser (LIF). Recientemente, los investigadores describieron un método simple en el cual el reactivo ATTO-TAG FQ se utiliza para derivar soluciones muy diluidas (10-8 M) de péptidos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Para utilizar con (aplicación)Detección de aminas primarias
Etiqueta o tinteATTO-TAG FQ
Cantidad10 mg
Fracción reactivaAldehído
Condiciones de envíoTemperatura ambiente
ObjetivoAmina primaria
FormularioMaciza
Línea de productosATTO-TAG
TipoReactivo de derivatización
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de –5 a –30 °C) y proteger de la luz.
Citations & References (18)
Citations & References
Abstract
Identification of proteins in single-cell capillary electrophoresis fingerprints based on comigration with standard proteins.
Journal:Anal Chem
PubMed ID:14570203
'In the previous paper in this Journal, we reported the use of capillary sieving electrophoresis to characterize proteins expressed by single cancer cells at specific phases in the cell cycle. Analysis of the data revealed one component with cell cycle-dependent changes in expression at the 99% confidence limit. However, the
Kinetics and apparent activation energy of the reaction of the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde with ovalbumin.
Journal:J Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID:12880858
'Incomplete labeling of proteins by a derivatizing reagent usually results in the formation of a large number of products, which can produce unacceptable band broadening during electrophoretic analysis. In this paper, we report on the reaction of the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde (FQ) with the lysine residues of ovalbumin. Mass spectrometry
Solid-phase fluorescent labeling reaction of picomole amounts of insulin in very dilute solutions and their analysis by capillary electrophoresis.
Journal:Electrophoresis
PubMed ID:7588523
'The fluorescent labeling of peptides at concentrations as low as 10(-8) M can be achieved by using a solid-phase reactor. Using oxidized insulin chain B as a test peptide, we demonstrate the use of an Immobilon CD membrane to capture and preconcentrate peptides. Insulin chain B can then be labeled
MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection.
Journal:J Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID:12880861
'MitoTracker Green (MTG) is a mitochondrial-selective fluorescent label commonly used in confocal microscopy and flow cytometry. It is expected that this dye selectively accumulates in the mitochondrial matrix where it covalently binds to mitochondrial proteins by reacting with free thiol groups of cysteine residues. Here we demonstrate that MTG can
Analysis of aminophospholipid molecular species by methyl-beta-cyclodextrin modified micellar electrokinetic capillary chromatography with laser-induced fluorescence detection.
Journal:Electrophoresis
PubMed ID:12207317
'Micellar electrokinetic capillary chromatography (MEKC) with laser-induced fluorescence detection is used for the analysis of three classes of aminophospholipids: phosphatidylethanolamine (PE), phosphatidylserine (PS), and lysophosphatidylethanolamine (LPE) molecular species. 3-(2-Furoyl) quinoline-2-carboxaldehyde (FQ), a fluorogenic dye, was employed for labeling of these phospholipids. The FQ-labeled lipid species were then separated by sodium