Collagen I, rat tail

Collagen I Rat Protein, Tail

Número de catálogo	Cantidad
A1048301	De 20 a 25 mL

Número de catálogo A1048301

Precio (USD)

502,20

Añadir al carro de la compra

Cantidad:

De 20 a 25 mL

Precio (USD)

502,20

Añadir al carro de la compra

Collagen I, Rat Tail is the most common fibrillar collagen (90%) found in skin, bone, tendons, and other connective tissues. It is supplied at ~4 mg/mL in 20 mM acetic acid, providing the flexibility to dilute to lower concentrations. (See Certificate of Analysis for exact concentration.) The detailed gelling procedure forms a firm, clear gel for 3D applications.

Collagen is a fibrous protein that consists of three α-chains that can combine to form a rope-like triple helix, providing tensile strength to the extracellular matrix (ECM) where it plays a key role in cell growth, differentiation, attachment, and cell migration. The α-chains contain GXY repeats. Glycine (G) is a small amino acid that fits well in the triple helix. X and Y are typically proline and hydroxyproline, which is critical for collagen stability. Type I is the most common fibrillar collagen (90%) and is mostly found in skin, bone, tendons, and other connective tissues.

Rat tail collagen I can be prepared as a clear gel providing a 3D matrix or surface coated on tissue culture plates as a substrate for culturing primary cells such as keratinocytes and hepatocytes.

For Research Use Only. Not for use in diagnostic procedures.

Especificaciones

Concentración4 mg/mL

Nivel de endotoxinas≤10 EU/mL, ≤ 10 EU/mL

Línea de productosGibco

Tipo de productoCollagen

Forma de proteínaNative

Subtipo de proteínaCollagen

CantidadDe 20 a 25 mL

Categoría de investigaciónExtracellular Matrix & Cell Adhesion

Condiciones de envíoWet Ice

Para utilizar con (aplicación)Cell Culture

FormularioLiquid

EspecieRat

Unit SizeEach

Contenido y almacenamiento

Store in refrigerator (2–8°C) and protect from light.

Preguntas frecuentes

How do the following compounds help cells attach in culture - collagen, gelatin, hydrated collagen, matrigel, poly-L-ornithine, poly-L-lysine, and poly-D-lysine?

The polystyrene that culture flasks are made of tends to have a negative charge. Many cell types will still attach because the plasticware is treated in a way to make them hydrophilic. However cell types that do not attach well to the plastic, or are grown in medium with little or no serum protein, will attach better if the plastic is coated with a protein/polymer which has an overall positive charge.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is the Collagen I, rat tail (Cat. No. A1048301) pepsin-digested?

No, the Collagen I, rat tail is not pepsin-digested.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you have an alternative for the discontinued Coating Matrix Kit Protein (Cat. No. R011K)?

Unfortunately, we do not have an animal-origin-free alternative for the Coating Matrix Kit Protein (Cat. No. R011K).However, we offer Rat or Bovine collagen that can be used as coating matrices. Please see the product pages linked below:

Collagen I, bovine (Cat. No. A1064401)

Collagen I, rat tail (Cat. No. A1048301)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer an alternative product for Collagen I, rat tail (Cat. No. A1048301), that can be prepared as a clear liquid gel for coating tissue culture plates?

An alternative for Collagen I, rat tail (Cat. No A1048301) is Collagen I, bovine (Cat. No A1064401). While the source of Collagen I is different, they can both be prepared as a clear gel providing a 3D matrix or surface coating on tissue culture plates as a substrate for culturing primary cells such as keratinocytes and hepatocytes.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I would like to use Collagen I Rat Protein, Tail (Cat. No. A1048301) for coating cell culture dishes. In the first step of the thin coating protocol, what should I use to dilute the 17.4 M acetic acid stock solution to 20 mM?

You can just use water to dilute the acetic acid stock solution to 20 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Collagen I, rat tail FAQs