Enzima TrypLE™ Select CTS™

Citas y referencias (1)

Gibco™

Enzima TrypLE™ Select CTS™

TrypLE™ Select CTS™, una alternativa sin origen animal a la tripsina porcina, es una enzima recombinante derivada de la fermentaciónMás información

Número de catálogo	Cantidad
A1285901	100 mL

Número de catálogo A1285901

Precio (USD)

98,31

Añadir al carro de la compra

Cantidad:

100 mL

Precio (USD)

98,31

Añadir al carro de la compra

TrypLE™ Select CTS™, una alternativa sin origen animal a la tripsina porcina, es una enzima recombinante derivada de la fermentación microbiana. La línea de productos GIBCO™ CTS™ le permite reducir la carga de cualificar los reactivos durante su transición de aplicaciones de investigación a aplicaciones clínicas.

•GIBCO™ TrypLE™ Select ™ CTS™ le permite reducir la carga de cualificar los reactivos durante su transición de aplicaciones de investigación a aplicaciones clínicas.
•Sin componentes de origen humano o animal
•Menor riesgo de contaminación vírica o por priones
•Cinética de eliminación y viabilidad de recuperación comparables a las de la tripsina porcina
•Demostración de la actividad en varias líneas de células, incluidas aquellas adheridas con firmeza
•Estable a temperatura ambiente (de 15 a 30 °C) durante hasta 24 meses.
•Fabricado con equipos específicos, esterilizados en autoclave o desechables.

TrypLE™ Select CTS™, una alternativa sin origen animal a la tripsina porcina, es una enzima recombinante derivada de la fermentación microbiana. Este material se utiliza para disociar las líneas de células dependientes de anclaje de los materiales de plástico. TrypLE Select ha demostrado la capacidad de disociar las células cultivadas en sistemas suplementados con y sin suero. El producto se suministra como una solución madre 1X formulada en DPBS con 1 mM de EDTA. La concentración de rProtease es exclusiva de
Invitrogen Corp.

Los productos GIBCO™ CTS™ son de alta calidad y se suministran con documentación armonizada, como certificados de análisis, certificados de origen y acceso a cartas de autorización de nuestros archivos principales sobre fármacos, según corresponda. La línea de productos GIBCO™ CTS™ le permite reducir la carga de cualificar los reactivos durante su transición de aplicaciones de investigación a aplicaciones clínicas.

Para su uso en investigación o fabricación de productos basados en células, genes o tejidos. PRECAUCIÓN: No se ha diseñado para la administración directa en seres humanos o animales

Para su uso en investigación o fabricación de productos basados en células, genes o tejidos. PRECAUCIÓN: no diseñado para la administración directa en seres humanos o animales.

Especificaciones

Tipo de célulaCélulas de mamíferos

Concentración1 X

Para utilizar con (aplicación)Investigación clínica, investigación traslacional

FormatoFrasco(s)

Características ecológicasEmbalaje sostenible

Calidad de fabricaciónISO 13485, MDSAP, FDA-registered, 21 CFR 820

Cantidad100 mL

Duración de almacenamiento24 meses

Condiciones de envíoTemperatura ambiente

ClasificaciónLibre de material de origen animal

FormularioLíquido

Tipo de productoReactivo para disociación de cultivo celular

EsterilidadEstéril con filtro

Sin aditivosSin rojo fenol

Unit SizeEach

Contenido y almacenamiento

Almacenar a temperatura ambiente.

Preguntas frecuentes

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?

There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For your TrypLE products, how does the enzyme get inactivated?

Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:

After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

View all Enzima TrypLE™ Select CTS™ FAQs

Citations & References (1)

Citations & References

Abstract

An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.

Authors:Nishishita N, Muramatsu M, Kawamata S

Journal:

PubMed ID:25973330

Culturing human Pluripotent Stem Cells (hPSC)s in chemically defined medium and feeder-free condition can facilitate metabolome and proteome analysis of culturing cells and medium, and reduce regulatory concerns for clinical application of cells. And in addition, if hPSC are passaged and cryopreserved in single cells it also facilitates quality control ... More