Pastillas inhibidoras de la proteasa Pierce™, sin EDTA
Pastillas inhibidoras de la proteasa Pierce™, sin EDTA
Thermo Scientific™

Pastillas inhibidoras de la proteasa Pierce™, sin EDTA

Las pastillas inhibidoras de proteasas Pierce, sin EDTA, contienen inhibidores de proteasas de amplio espectro que son altamente eficaces paraMás información
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Número de catálogoCantidad
A3296520 pastillas
Número de catálogo A32965
Precio (USD)
599,40
Each
Añadir al carro de la compra
Cantidad:
20 pastillas
Precio (USD)
599,40
Each
Añadir al carro de la compra
Las pastillas inhibidoras de proteasas Pierce, sin EDTA, contienen inhibidores de proteasas de amplio espectro que son altamente eficaces para prevenir la degradación proteolítica durante experimentos de lisis celular y extracción de proteínas.

Características de las pastillas inhibidoras de proteasas sin EDTA:
Formulación mejorada: las pastillas se disuelven rápidamente para formar una solución transparente
Económicas: más rentables que otras formulaciones disponibles comercialmente para volúmenes equivalentes
Prácticas: formulaciones de amplio espectro, completamente públicas y listas para su uso
Compatibles: se utilizan directamente en ensayos BCA Pierce
Sin EDTA: formuladas sin EDTA, un inhibidor de la metaloproteinasa (solo para formulaciones sin EDTA)

Las pastillas inhibidoras de proteasas Pierce protegen las proteínas de la degradación proteolítica a través de la inhibición de proteasas serinas, proteasas cisteínas, proteasas ácido aspárticas y aminopeptidasas que normalmente están presentes en las muestras de lisado celular.Las pastillas contienen AEBSF, aprotinina, bestatina, E-64, leupeptina y pepstatina A. Es estable durante el almacenamiento a 4 °C durante 12 meses, cada pastilla es suficiente para 50 ml o de la solución, y es adecuada para la preparación de reactivos antes de la extracción de proteínas a partir de tejidos y células cultivadas.

La proteasas conforman una gran categoría de enzimas, incluidas las endopeptidasas y exopeptidasas, que catalizan la descomposición hidrolítica de las proteínas en péptidos o aminoácidos.Las proteasas residen en compartimentos celulares, pero tras la lisis celular, las proteasas se liberan, lo que resulta en la degradación de proteínas valiosas.Por esta razón, se suelen añadir múltiples compuestos conocidos para inactivar o inhibir las proteasas a los tampones de lisis celular o extractos celulares.

Para usar las pastillas inhibidoras de la proteasa Pierce, simplemente disuelva 1 comprimido en 50 ml de tampón o lisado.La fórmula inhibidora de proteasa es compatible con la mayoría de los reactivos de lisis celular basados en detergentes, incluidas las soluciones para lisis celular Pierce.Las pastillas inhibidoras de proteasa Pierce no contienen inhibidores de la fosfatasa.Si es necesario, se pueden añadir inhibidores de fosfatasas, como las pastillas inhibidoras de fosfatasas Pierce, junto con la pastilla inhibidora de proteasas.

Productos relacionados
Pastillas inhibidoras de proteasas Pierce
Minipastillas inhibidoras de proteasas Pierce, sin EDTA
Minipastillas inhibidoras de fosfatasas Pierce
Minipastillas inhibidoras de proteasas y fosfatasas Pierce

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
InhibidoresInhibidor de proteasa
Cantidad20 pastillas
Condiciones de envíoTemperatura ambiente
Suficiente paraSolución de 10 ml
FormularioSólido
Línea de productosPierce
Tipo de productoCóctel inhibidor de proteasa
Unit SizeEach
Contenido y almacenamiento
1 vial, almacenar entre 2 °C y 8 °C

Preguntas frecuentes

Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What formats of protease inhibitors do you offer?

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer any of the components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets individually?

Yes, the following components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are offered separately:

- AEBSF (Cat. No. 78431)
- Aprotinin (Cat. No. 78432)
- Bestatin (Cat. No. 8433)
- E-64 (Cat. No. 78434)
- Leupeptin (Cat. No. 78435)
- Pepstatin A (Cat. No. 78436)
- EDTA (Cat. No. 17892).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the protease and phosphatase inhibitor tablets and mini tablets?

The final concentration of each component in the protease and phosphatase inhibitor tablets and mini tablets is proprietary. We release only the identities of the inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I store the reconstituted solution of protease, phosphatase and (protease and phosphatase) inhibitor tablet or mini tablet and if so, for how long?

The Pierce protease, phosphatase, as well as (protease and phosphatase) inhibitor tablets and mini tablets are stable for at least one year when stored at 4 degrees C, but we only recommend preparing fresh solutions just before needed. If you are interested in liquid protease or phosphatase inhibitors, please use our inhibitor cocktails (https://www.thermofisher.com/search/results?query=protease%20inhibitor%20cocktails&focusarea=Search%20All).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (3)

Citations & References
Abstract
An Introductory Guide to Protease Sensitive Linker Design Using Matrix Metalloproteinase 13 as an Example.
Authors:Hamm P,Meinel L,Driessen MD
Journal:ACS biomaterials science & engineering
PubMed ID:38813796
Proteases play a crucial role, not only in physiological, but also in pathological processes, such as cancer, inflammation, arthritis, Alzheimer's, and infections, to name but a few. Their ability to cleave peptides can be harnessed for a broad range of biotechnological purposes. To do this efficiently, it is essential to ... More
Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions.
Authors:Wang K,Escobar M,Li J,Mahata B,Goell J,Shah S,Cluck M,Hilton IB
Journal:Nucleic acids research
PubMed ID:35849129
Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful technologies to synthetically reshape the human epigenome and gene expression. Despite the increasing adoption of these platforms, their relative potencies and mechanistic differences are incompletely characterized, particularly at human enhancer-promoter pairs. Here, we systematically compared the most widely adopted dCas9-based transcriptional activators, ... More
Not just fat: investigating the proteome of cetacean blubber tissue.
Authors:Kershaw JL,Botting CH,Brownlow A,Hall AJ
Journal:Conservation physiology
PubMed ID:29479430
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This ... More