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Suero bovino fetal, aprobado por el sistema de Tet, regiones aprobadas por el USDA
Gibco™

Suero bovino fetal, aprobado por el sistema de Tet, regiones aprobadas por el USDA

El FBS aprobado por el sistema de Tet Gibco está formulado para aplicaciones de cultivo celular utilizando sistemas de expresiónMás información
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Número de catálogoCantidadFormato
A473630150 mLFrasco
A4736401500 mLFrasco
Número de catálogo A4736301
Precio (USD)
213,84
Each
Cantidad:
50 mL
Formato:
Frasco
Precio (USD)
213,84
Each
El FBS aprobado por el sistema de Tet Gibco está formulado para aplicaciones de cultivo celular utilizando sistemas de expresión de genes regulados por tetraciclina y derivados de tetraciclina. Los ensayos basados en células que detectan la presencia de tetraciclina y de análogos de tetraciclina se utilizan para seleccionar el FBS que proporciona el rango más alto de inducción.

• Diseñado para garantizar la ausencia de tetraciclina detectable y sus derivados que pueden interferir con la regulación exacta de la expresión génica
• Las pruebas funcionales proporcionan la confianza de que la expresión génica de tetracilina no deseada no dará lugar
• Categoría de Sera: especialidad

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Tipo de célulaUso para una amplia variedad de tipos de células, especialmente líneas celulares sensibles
Certificaciones/conformidadCertificación ISO 13485
ClasificaciónValor
Concentración de endotoxinas< 50 EU/mL (niveles habituales de <10 EU/mL)
GradoValor
Características ecológicasEmbalaje sostenible
Concentración de hemoglobina<25 mg/dL
Línea de productosOne Shot
Grado de pureza o calidadValor
Duración de almacenamiento3 años
Condiciones de envíoCongelado
Tipo especializadoAprobado por el sistema de Tet
EspecieGanado/bovino
Probado para50 pruebas de especificación de calidad, incluyendo 9 pruebas de virus CFR, endotoxinas y rendimiento
AgeFetal
País de origenMéxico
FormatoFrasco
Tipo de productoSuero fetal bovino
Cantidad50 mL
EsterilidadEstéril
Sterilization MethodTriple filtrado, 0,1 μm
Unit SizeEach
Contenido y almacenamiento
<-10°C

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Why do you use a cell-based assay instead of HPLC to determine the tetracycline concentration/presence in Fetal Bovine Serum, Tet system approved?

While competitors may use HPLC or other assays to determine the presence of tetracycline, we developed a cell-based assay because we wanted to verify that the tetracycline present, if any, in this product would not be enough to alter the experiment. The only way to confirm this is to actually demonstrate the efficacy of the serum in a dish with cells (cell-based assay).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How is Fetal Bovine Serum, Tet system approved verified to be tetracycline-free?

Fetal Bovine Serum, Tet system approved is not tetracycline-free, and no serum can be absolutely confirmed to be tetracycline-free without altering the performance of the product. We developed a cell-based assay to confirm that tetracycline, if present, is not in high enough concentrations to alter Tet experiments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer Fetal Bovine Serum, Tet system approved that has already been heat inactivated?

Sorry, we do not offer Fetal Bovine Serum, Tet system approved that has already been heat inactivated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it normal to have precipitation in Fetal Bovine Serum, Tet system approved?

Since our sera are not pre-aged before filtration, turbidity or flocculent debris may develop upon thawing or storage. This flocculence is a normal characteristic of the serum and does not adversely affect performance of the serum.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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