Fetal Bovine Serum, Premium
Fetal Bovine Serum, Premium
Fetal Bovine Serum, Premium
Fetal Bovine Serum, Premium
Fetal Bovine Serum, Premium
Gibco™

Fetal Bovine Serum, Premium

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Gibco fetal bovine sera offer an excellent value for basic cell culture, specialty research, and specific assays.
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Número de catálogoCantidadFormatoInactivado por calorGamma-irradiated
A5670701500 mLFrascoNoNo
A5670201500 mLFrascoSíSí
A567040150 mLOne ShotNoNo
A567040210 x 50 mLOne ShotNoNo
A567050150 mLOne ShotSíNo
A567050210 x 50 mLOne ShotSíNo
A5670801500 mLFrascoSíNo
A5670901500 mLFrascoNoSí
Número de catálogo A5670701
Precio (USD)
1.250,64
Each
Añadir al carro de la compra
Cantidad:
500 mL
Formato:
Frasco
Inactivado por calor:
No
Gamma-irradiated:
No
Precio (USD)
1.250,64
Each
Añadir al carro de la compra

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements.

Sera category: Premium

  • Use for general cell culture with common cell lines
  • Low endotoxin and hemoglobin levels
  • Endotoxin level: <10 EU/mL
  • Hemoglobin level: <25 mg/dL
  • Origin: refer to CoA for specific origin. All products may not be available in all regions due to importation regulations.
  • US Department of Agriculture (USDA) safety tested for the absence of bluetongue and akabane from countries that are USDA-approved for importation into the US

Gibco serum delivers

  • ISO 13485–certified; processed in FDA registered facilities
  • Triple-filtered at 0.1 μm
  • Gibco bottle easier to use in hood; helps reduce risk of contamination and improve consistency
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

* FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Concentración de endotoxinas≤10 EU/mL
Para utilizar con (aplicación)*FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.
Gamma-irradiatedNo
GradoPremium
Características ecológicasLess waste
Inactivado por calorNo
Concentración de hemoglobina≤25 mg/dL
Grado de pureza o calidadPremium
Duración de almacenamiento5 Years
Condiciones de envíoDry Ice
EspecieCattle/Bovine
Probado paraUp to 60 quality tests including 9CFR virus testing, endotoxin, and performance
AgeFetal
País de origenConsulte CoA para conocer el origen específico*
FormularioFrozen
FormatoFrasco
Tipo de productoFetal Bovine Serum
Cantidad500 mL
EsterilidadEstéril con filtro
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
Contenido y almacenamiento
Store at ≤-10°C.

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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