Solución de proteinasa K recombinante (20 mg/ml)
Solución de proteinasa K recombinante (20 mg/ml)
Invitrogen™

Solución de proteinasa K recombinante (20 mg/ml)

La proteinasa K recombinante es una serina proteasa inespecífica que resulta útil para la digestión general de proteínas.Más información
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Número de catálogoCantidad
AM25485 x 1,25 mL
AM25461,25 mL
Número de catálogo AM2548
Precio (USD)
460,41
Each
Añadir al carro de la compra
Cantidad:
5 x 1,25 mL
Precio (USD)
460,41
Each
Añadir al carro de la compra
La proteinasa K recombinante es una serina proteasa inespecífica que resulta útil para la digestión general de proteínas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Especie del huéspedT. album
Tipo de productoProteinase K
Cantidad5 x 1,25 mL
Condiciones de envíoHielo seco
Concentración20 mg/ml
FormularioLíquido
Línea de productosAmbion
Unit SizeEach
Contenido y almacenamiento
-20 °C

Preguntas frecuentes

How do I isolate DNA from single bacterial colonies before PCR?

Scrape a single colony from the surface of an agar plate and transfer to 12 µL of SCL solution [10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 50 µg/mL proteinase K] and incubate for 15 min at 55 degrees C. Inactivate proteinase K for 15 min at 80 degrees C and add 20 µL of deionized water. Centrifuge at 12,000 x g for 3 min. Transfer the supernatant to a new tube.

What are the recommended conditions for proteinase K treatment when isolating RNA or DNA samples?

Concentration: Generally proteinase K is used in the concentration range of 50 to 500 µg/mL at 65 degrees C in the presence of SDS (0.5-1%).

Temperature optimum: 65 degrees C; 12X more active at 65 degrees C than at 25 degrees C.

pH: Proteinase K is stable over a wide pH range (4.0 to 12.5), with optimal activity at pH 6.5 to 9.5. It is most stable at pH 8.
Inactivation: Heat inactivate Proteinase K at 80 degrees C for 15 min. Phenol extraction is the recommended method to ensure complete inactivation.

Inhibited by: PMSF (0.1 to 1.0 mM of PMSF is usually sufficient for inhibition of Proteinase K)

Not inhibited by: Proteinase K is not inactivated by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents or by trypsin or chymotrypsin inhibitors. Activity can be stimulated by addition of denaturing agents (SDS and urea).

I received Recombinant Proteinase K Solution (Cat. No. AM2546, AM2548) on Friday and it sat over the weekend and thawed. Is it still usable?

No, we do not recommend using it.

Do you offer the Proteinase K from the MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828) separately from the kit?

We do not sell the Proteinase K from the MagMAX mirVana Total RNA Isolation Kit as a standalone item. A different Proteinase K product (Cat. No. AM2548) can be purchased as a standalone item and can be used at 2.5X in the MagMAX mirVana protocol if you would like to add more Proteinase K to the procedure.