Acetato de amonio (5 M), sin ARNasa
Acetato de amonio (5 M), sin ARNasa
Invitrogen™

Acetato de amonio (5 M), sin ARNasa

La solución de acetato de amonio 5 M de calidad para biología molecular Ambion® se suministra en un frasco deMás información
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Número de catálogoCantidad
AM9071500 mL
AM9070G100 mL
Número de catálogo AM9071
Precio (USD)
208,98
Each
Añadir al carro de la compra
Cantidad:
500 mL
Precio (USD)
208,98
Each
Añadir al carro de la compra
La solución de acetato de amonio 5 M de calidad para biología molecular Ambion® se suministra en un frasco de 500 ml. La solución cuenta con certificación de ausencia de ARNasa, es económica y está lista para su uso. Debido a la omnipresencia de ARNasa, la fabricación de productos para su uso con ARN es especialmente complicada. Los reactivos y tampones sin nucleasas de Ambion® se fabrican en instalaciones diseñadas específicamente para impedir la introducción de nucleasas. Los ensayos de ARNasa de alta sensibilidad se realizan en varias etapas diferentes del proceso de fabricación para garantizar la máxima calidad. Estos reactivos se someten a rigurosas pruebas de contaminación de endonucleasas no específicas, exonucleasas y actividad de ARNasa.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Nombre del producto químico o materialAcetato de amonio
Tipo de empaquetadoFrasco
Línea de productosAmbion
PurezaGrado de biología molecular
Cantidad500 mL
Condiciones de envíoTemperatura ambiente
Concentración5 M
FormularioLíquido
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente.

Preguntas frecuentes

What salts can be used to precipitate total RNA? What are the advantages of each? Do they require ethanol?

The three salts that can be used are:

- Guanidine thiocyanate, which requires ethanol. Guanidine thiocyanate is a common agent used for isolating RNA and we recommend it especially for tissues high in ribonuclease activity, such as the pancreas or spleen.
- Ammonium acetate, which requires ethanol. Ammonium acetate is useful when reducing coprecipitation of unwanted dNTPs and oligosaccharides. However, it should not be used when the nucleic acid will be phosphorylated using T4 polynucleotide kinase, since this enzyme is inhibited by ammonium ions.
- Lithium chloride, which does not require ethanol. LiCl is very effective in precipitating RNA but does not efficiently precipitate protein or DNA. It also does not precipitate unincorporated nucleotides.

How can I concentrate DNA solution using ethanol precipitation?

Ethanol precipitation is frequently used for concentration of DNA solutions and for removal of protein, salt, and unincorporated nucleotides. The two most common protocols use either 0.3 M sodium acetate (0.1 volume of 3 M) or 2.5 M ammonium acetate (0.5 volume of 7.5 M), along with 2 to 2.5 volumes of ethanol. Studies at Thermo Fisher Scientific (1, 2) have shown these two salts to be equally effective for recovery of small amounts of DNA from small volumes and for removal of unincorporated nucleotides from labeling reactions.

DNA was found to precipitate readily with room temperature ethanol and room temperature centrifugation. For DNA concentrations >0.1 µg/mL, no incubation period is required. For improved recovery of DNA from dilute solutions (10 ng/mL), overnight incubation in the ethanol and extended (30 min) centrifugation is recommended. Addition of ammonium acetate to 2.5 M (without ethanol) has also been shown to be effective in precipitating proteins while leaving the DNA in solution (2).

1. Zeugin, J.A. and Hartley, J.L. (1985) FOCUS 7:4, 1.

2. Crouse, J. and Amorese, D. (1987) FOCUS 9:2, 3.