GlowQuant™ ATP Cell Viability Assay Kit

The GlowQuant™ ATP Cell Viability Assay Kit is a ready-to-use, bioluminescence-based solution for rapid and sensitive measurement of viable cells through ATP detection and quantification. Designed for wide variety of applications, this luminescence-based ATP assay supports cell health, proliferation studies, and cytotoxicity analysis with a simple one-step workflow.

The assay uses firefly luciferase to catalyze the oxidation of luciferin in the presence of ATP and oxygen, generating a stable bioluminescent signal directly proportional to ATP concentration, and therefore to the number of viable cells.

By measuring intracellular ATP (a universal marker of metabolically active cells), this assay provides a direct and quantitative assessment of cell health.

Key Features:

Features of GlowQuant™ ATP Cell Viability Assay Kit include:

• High sensitivity ATP detection— Detects low cell numbers, down to ~50 cells/well in a 96-well plate.
• Fast and simple — One-step add-and-read format with minimal hands-on time and results in as little as 10 minutes
• No reagent preparation required — Supplied as a ready-to-use, pre-formulated reagent.
• Stable signal — Bioluminescent glow with a signal half-life > 2 hours for flexible plate reading.
• Quantitative and reliable performance — Linear response over a wide dynamic range for reliable cell-number estimation.
• Microplate reader compatible — Designed for use with standard luminescence microplate readers in 96- and 384-well formats; compatible with automation.

How it Works

The GlowQuant™ assay features a simple add-and-read protocol with no separate lysis step required.

1. Add an equal volume of reagent directly to cultured cells.
2. Mix for 1 minute.
3. Incubate at room temperature for 10–20 minutes.
4. Measure luminescence using a luminometer or compatible microplate reader.

The emitted luminescent signal is proportional to intracellular ATP levels. Because viable cells maintain ATP while damaged or non-viable cells rapidly lose ATP, signal intensity directly reflects the number of metabolically active cells in each well.

Supporting Your Cell Health Research Across Detection Modes

Thermo Fisher Scientific supports cell health research with a broad portfolio of validated assays spanning luminescence, fluorescence, and absorbance detection modes. From rapid ATP-based viability assays to multiplex-compatible fluorescent and colorimetric solutions, our cell health tools are designed to integrate seamlessly with standard plate readers, automated workflows, and high-throughput screening platforms— to help provide scalable solutions backed by global technical support.