Tampón de transferencia Bolt™ (20X)
Tampón de transferencia Bolt™ (20X)
Invitrogen™

Tampón de transferencia Bolt™ (20X)

El tampón de transferencia Bolt (20X) se usa para transferir proteínas de los geles Bolt Bis-Tris Plus a membranas paraMás información
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Número de catálogoCantidad
BT000611 l
BT0006125 ml
Número de catálogo BT00061
Precio (USD)
-
Cantidad:
1 l
El tampón de transferencia Bolt (20X) se usa para transferir proteínas de los geles Bolt Bis-Tris Plus a membranas para la transferencia Western. Mantiene el entorno de pH neutro establecido durante la electroforesis. El pH neutro protege contra la modificación de las cadenas laterales de aminoácidos y es compatible con la secuenciación de proteínas N-terminal mediante la degradación de Edman.

El antioxidante Bolt se puede utilizar con el tampón de transferencia Bolt para mejorar la transferencia de proteínas reducidas a las membranas.

Consulte todos los tampones y reactivos disponibles para SDS-PAGE

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
TampónTampones de transferencia
Concentración20 X
FormularioLíquido
Compatibilidad del gelGeles Bolt™ Bis-Tris Plus
Línea de productosBolt
Tipo de productoTampón de transferencia Western Blot
Cantidad1 l
Volumen (métrico)1 l
Unit SizeEach
Contenido y almacenamiento
Almacenar entre 4 y 25 °C

Preguntas frecuentes

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

View all Tampón de transferencia Bolt™ (20X) FAQs