5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate
5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate
Citas y referencias (287)
Invitrogen™

5-(and-6)-Carboxy SNARF™-1, Acetoxymethyl Ester, Acetate

Carboxi SNARF™-1, acetato de éster acetoximetil, es un indicador de pH que penetra en la célula que tiene un pKaMás información
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Número de catálogoCantidad
C127220 x 50 μg
Número de catálogo C1272
Precio (USD)
1.075,68
Each
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Cantidad:
20 x 50 μg
Precio (USD)
1.075,68
Each
Añadir al carro de la compra
Carboxi SNARF™-1, acetato de éster acetoximetil, es un indicador de pH que penetra en la célula que tiene un pKa de ∼7,5 despés de la esterificación; por lo tanto, es útil para medir los cambios de pH entre pH 7 y pH 8. Carboxi-SNARF™-1 muestra un cambio significativo de emisión dependiente del pH de la fluorescencia amarilla-naranja a la fluorescencia roja profunda en condiciones ácidas y básicas, respectivamente Esta dependencia del pH permite utilizar la relación de las intensidades de fluorescencia del colorante en dos longitudes de onda de emisión, normalmente 580 nm y 640 nm, para las determinaciones cuantitativas del pH

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Método de detecciónFluorescente
Tipo de coloranteIndicador de pH
Cantidad20 x 50 μg
Condiciones de envíoTemperatura ambiente
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo, Lector de microplacas
Línea de productosSNARF
Tipo de productoIndicador de pH
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador entre -5 °C y -30 °C

Preguntas frecuentes

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (287)

Citations & References
Abstract
Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.
Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM
Journal:The American journal of physiology
PubMed ID:8967445
Molecular cloning and functional expression of a rat Na+/H+ exchanger (NHE5) highly expressed in brain.
Authors:Attaphitaya S, Park K, Melvin JE
Journal:J Biol Chem
PubMed ID:9933642
We report here the cloning, primary structure, heterologous expression, tissue distribution, and localization of a cDNA encoding rat NHE5, a fifth member of the mammalian plasma membrane Na+/H+ exchanger (NHE) gene family. The full-length open reading frame as well as 34 nucleotides of 5'-untranslated and 1443 nucleotides of 3'-untranslated sequences ... More
Measurement of intracellular pH.
Authors:Boyer MJ, Hedley DW
Journal:Methods Cell Biol
PubMed ID:7532259
Simultaneous imaging of intracellular [Ca2+] and pH in single MDCK and glomerular epithelial cells.
Authors:Wiegmann TB, Welling LW, Beatty DM, Howard DE, Vamos S, Morris SJ
Journal:Am J Physiol
PubMed ID:8238309
The interrelationships between changes in intracellular calcium concentration ([Ca2+]i) and intracellular pH in Madin-Darby canine kidney cells and kidney glomerular epithelial cells exposed to various stimuli were analyzed simultaneously using a new design of a fluorescence video microscope. Cells were double labeled with indo 1 and SNARF 1 dyes and ... More
Identification of band 3-like proteins and Cl-/HCO3- exchange in isolated cardiomyocytes.
Authors:Pucéat M, Korichneva I, Cassoly R, Vassort G
Journal:J Biol Chem
PubMed ID:7836397
'The identification of the protein that exerts the function of Cl-/HCO3- exchange is still unresolved in cardiac tissue. We have addressed this issue by using a multiple technical approach. Western blotting analysis with an antibody raised against human erythroid whole band 3 protein, the so-called protein that mediates the Cl-/HCO3- ... More
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