Sondas fluorescentes CellTracker™
Sondas fluorescentes CellTracker™
Citas y referencias (106)
Invitrogen™

Sondas fluorescentes CellTracker™

Es un colorante fluorescente adecuado para supervisar el movimiento de las células o su ubicación.
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Número de catálogoCantidadTipo de colorante
C29271 mgCellTracker Orange CMTMR
C702520 x 50 μgCellTracker Green CMFDA
C29251mgCellTracker Green CMFDA
C3455220 × 50 μgCellTracker™ Red CMTPX
Número de catálogo C2927
Precio (USD)
691,74
Each
Añadir al carro de la compra
Cantidad:
1 mg
Tipo de colorante:
CellTracker Orange CMTMR
Precio (USD)
691,74
Each
Añadir al carro de la compra
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

  • Este colorante se conserva bien, lo que permite el seguimiento multigeneracional de movimientos celulares.
  • Los espectros de excitación/emisión verde (492/517 nm maxima) son ideales para el multiplexing con proteínas y colorantes rojo fluorescente.
  • Fácil de usar: retire el medio, añada el colorante, incube 30 minutos y obtenga la imagen de las células.
  • Retención de la señal fluorescente superior a >72 horas (normalmente de tres a seis generaciones).
  • Baja citotoxicidad: no afecta a la viabilidad ni a la proliferación
  • El tinte fluorescente de CMAC CellTracker Blue se ha diseñado para atravesar libremente las membranas celulares hasta las células donde se transforma en productos de reacción impermeabilizantes de membranas celulares.
  • El colorante se transfiere a las células hijas, pero no a las celdas adyacentes de una población.
  • Estable, no tóxico a las concentraciones de trabajo, bien conservado en las células, y brillante fluorescente a pH fisiológico

Adhesión celular, análisis celular, proliferación celular, marcado y seguimiento celular, viabilidad celular y citotoxicidad, viabilidad celular, proliferación y función, Imágenes celulares, ensayos de toxicología celular, quimiotaxis y migración celular, descubrimiento y desarrollo de fármacos, marcado celular general, detección de glutatión, detección de alto contenido (HCS), inmunofluorescencia (IF), tinción y detección de inmunofluorescencia, señalización y homeostomía, señalización, identificación y señalización Seguimiento microbiano, estrés nitrooxidativo, ensayos ADME/Tox basados en dianas, detección de pH

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorNaranja
DescripciónCellTracker™ Orange CMTMR Dye, 1 mg
Tipo de coloranteCellTracker Orange CMTMR
Emisión565 nm
Intervalo de longitud de onda de excitación541 nm
FormularioDry Powder
Línea de productosCellTracker
Cantidad1 mg
Tipo de reactivoCompuestos de seguimiento celular, reactivos de etiquetado celular
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaOtras etiquetas o colorantes
Tipo de productoTinte
SubCellular LocalizationCytoplasm
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to stain my cells with CellTracker dyes or CFDA SE, but I'm not seeing much signal. What can I do?

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I like how calcein dyes label the whole cell. How long can I track my cells with them, and can I fix them?

Calcein dyes diffuse into cells, the 'AM' moiety is cleaved by cellular esterases and then are observed in the cytoplasm without binding to anything. This provides a 'whole cell' label. Calcein dyes may be pumped out by normal cellular efflux mechanisms, sometimes within a very short time, especially for cell types that may exhibit drug resistance, unless the efflux is inhibited (such as with probenecid). The dyes are not crosslinked with aldehyde-based fixation, unlike protein-binding CellTracker dyes, and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dyes from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

View all Sondas fluorescentes CellTracker™ FAQs

Citations & References (106)

Citations & References
Abstract
Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.
Authors:Van Amersfoort ES, Van Strijp JA
Journal:Cytometry
PubMed ID:7875036
A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More
Single-molecule analysis of cadherin-mediated cell-cell adhesion.
Authors:Panorchan P, Thompson MS, Davis KJ, Tseng Y, Konstantopoulos K, Wirtz D
Journal:J Cell Sci
PubMed ID:16371651
'Cadherins are ubiquitous cell surface molecules that are expressed in virtually all solid tissues and localize at sites of cell-cell contact. Cadherins form a large and diverse family of adhesion molecules, which play a crucial role in a multitude of cellular processes, including cell-cell adhesion, motility, and cell sorting in ... More
Inhibition of angiogenesis and metastases of the Lewis-lung cell carcinoma by the quinoline-3-carboxamide, Linomide.
Authors:Borgström P, Torres Filho IP, Hartley-Asp B
Journal:Anticancer Res
PubMed ID:7544090
'Linomide has antitumor effects when administered in vivo but not in vitro. Recent data indicate that at least part of this effect can be attributed to anti-angiogenic properties. The aim of the present investigation was to study the anti-angiogenic effects of Linomide on early tumor-induced angiogenesis in vivo, using a ... More
Stochastic regulation of cell migration from the efferent lymph to oxazolone-stimulated skin.
Authors:West CA, He C, Su M, Rawn J, Swanson S, Hay JB, Mentzer SJ
Journal:J Immunol
PubMed ID:11160191
'The systemic immune response is a dynamic process involving the trafficking of lymphocytes from the Ag-stimulated lymph node to the peripheral tissue. Studies in sheep have demonstrated several phases of cell output in the efferent lymph after Ag stimulation. When skin contact sensitizers are used as Ag, the efferent lymph ... More
T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases.
Authors:Mempel TR, Henrickson SE, Von Andrian UH
Journal:Nature
PubMed ID:14712275
'Primary T-cell responses in lymph nodes (LNs) require contact-dependent information exchange between T cells and dendritic cells (DCs). Because lymphocytes continually enter and leave normal LNs, the resident lymphocyte pool is composed of non-synchronized cells with different dwell times that display heterogeneous behaviour in mouse LNs in vitro. Here we ... More
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