Sondas fluorescentes CellTracker™

Citas y referencias (231)

Invitrogen™

Sondas fluorescentes CellTracker™

Name: Sondas fluorescentes CellTracker™
SKU: C7025
Price: 725.20 USD

Es un colorante fluorescente adecuado para supervisar el movimiento de las células o su ubicación.

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Número de catálogo	Cantidad	Tipo de colorante
C7025	20 x 50 μg	CellTracker Green CMFDA
C2925	1mg	CellTracker Green CMFDA
C2927	1 mg	CellTracker Orange CMTMR
C34552	20 × 50 μg	CellTracker™ Red CMTPX

4 opciones

Número de catálogo C7025

Precio (USD)

725,20

Each

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Cantidad:

20 x 50 μg

Tipo de colorante:

CellTracker Green CMFDA

Precio (USD)

725,20

Each

Añadir al carro de la compra

Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

Este colorante se conserva bien, lo que permite el seguimiento multigeneracional de movimientos celulares.
Los espectros de excitación/emisión verde (492/517 nm maxima) son ideales para el multiplexing con proteínas y colorantes rojo fluorescente.
Fácil de usar: retire el medio, añada el colorante, incube 30 minutos y obtenga la imagen de las células.
Retención de la señal fluorescente superior a >72 horas (normalmente de tres a seis generaciones).
Baja citotoxicidad: no afecta a la viabilidad ni a la proliferación
El tinte fluorescente de CMAC CellTracker Blue se ha diseñado para atravesar libremente las membranas celulares hasta las células donde se transforma en productos de reacción impermeabilizantes de membranas celulares.
El colorante se transfiere a las células hijas, pero no a las celdas adyacentes de una población.
Estable, no tóxico a las concentraciones de trabajo, bien conservado en las células, y brillante fluorescente a pH fisiológico

Adhesión celular, análisis celular, proliferación celular, marcado y seguimiento celular, viabilidad celular y citotoxicidad, viabilidad celular, proliferación y función, Imágenes celulares, ensayos de toxicología celular, quimiotaxis y migración celular, descubrimiento y desarrollo de fármacos, marcado celular general, detección de glutatión, detección de alto contenido (HCS), inmunofluorescencia (IF), tinción y detección de inmunofluorescencia, señalización y homeostomía, señalización, identificación y señalización Seguimiento microbiano, estrés nitrooxidativo, ensayos ADME/Tox basados en dianas, detección de pH

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorVerde

DescripciónCellTracker™ Green CMFDA Dye, 20 x 50 μg

Método de detecciónFluroescent probes

Tipo de coloranteCellTracker Green CMFDA

Emisión517 nm

Intervalo de longitud de onda de excitación492⁄517

Para utilizar con (aplicación)Fluroescence microscopy, cells in suspension, adherent cells

FormularioDry Powder

Peso molecular464.86

Línea de productosCellTracker

Cantidad20 x 50 μg

Tipo de reactivoCompuestos de seguimiento celular, reactivos de etiquetado celular

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaOtras etiquetas o colorantes

Tipo de productoTinte

SubCellular LocalizationCytoplasm

Unit SizeEach

Contenido y almacenamiento

Almacenar en congelador entre -5 °C y -30 °C

Preguntas frecuentes

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

What the shelf life for CellTracker Green CMFDA Dye (Cat. No. C7025, C2925)?

The shelf life for CellTracker Green CMFDA Dye is one year after receiving it, when stored desiccated at -20 degrees C.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Sondas fluorescentes CellTracker™ FAQs

Citations & References (231)

Citations & References

Abstract

In situ image analysis of interactions between normal human keratinocytes and fibroblasts cultured in three-dimensional fibrin gels.

Authors:Sun T,Haycock J,Macneil S

Journal:Biomaterials

PubMed ID:16510181

A point mutation in the binding subunit of a retroviral envelope protein arrests virus entry at hemifusion.

Authors:Zavorotinskaya T, Qian Z, Franks J, Albritton LM

Journal:J Virol

PubMed ID:14671127

The transmembrane subunits of viral envelope proteins are thought to perform all of the functions required for membrane fusion during entry of enveloped viruses. However, changes in a conserved SPHQ motif near the N terminus of the receptor binding subunit of a murine leukemia virus (MLV) envelope protein block infection ... More

Intercellular spreading of Porphyromonas gingivalis infection in primary gingival epithelial cells.

Authors:Yilmaz O, Verbeke P, Lamont RJ, Ojcius DM

Journal:Infect Immun

PubMed ID:16369027

'Porphyromonas gingivalis, an important periodontal pathogen, is an effective colonizer of oral tissues. The organism successfully invades, multiplies in, and survives for extended periods in primary gingival epithelial cells (GECs). It is unknown whether P. gingivalis resides in the cytoplasm of infected cells throughout the infection or can spread to ... More

Increased apical insertion of the multidrug resistance protein 2 (MRP2/ABCC2) in renal proximal tubules following gentamicin exposure.

Authors:Notenboom S, Wouterse AC, Peters B, Kuik LH, Heemskerk S, Russel FG, Masereeuw R,

Journal:J Pharmacol Exp Ther

PubMed ID:16757538

'Multidrug resistance protein (MRP) 2 (MRP2; ABCC2), an organic anion transporter apically expressed in liver, kidney, and intestine, plays an important protective role through facilitating the efflux of potentially toxic compounds. We hypothesized that upon a toxic insult, MRP2 is up-regulated in mammalian kidney, thereby protecting the tissue from damage. ... More

Apoptosis of adherent cells by recruitment of caspase-8 to unligated integrins.

Authors:Stupack DG, Puente XS, Boutsaboualoy S, Storgard CM, Cheresh DA

Journal:J Cell Biol

PubMed ID:11684710

'Integrin-mediated adhesion promotes cell survival in vitro, whereas integrin antagonists induce apoptosis of adherent cells in vivo. Here, we demonstrate that cells adherent within a three-dimensional extracellular matrix undergo apoptosis due to expression of unligated integrins, the beta subunit cytoplasmic domain, or its membrane proximal sequence KLLITIHDRKEF. Integrin-mediated death requires ... More

View all Sondas fluorescentes CellTracker™ citations