Ensayo de proliferación celular CyQUANT™ NF

Citas y referencias (65)

Invitrogen™

Ensayo de proliferación celular CyQUANT™ NF

Name: Ensayo de proliferación celular CyQUANT™ NF
SKU: C7026
Price: 741.96 USD

El kit de ensayo de proliferación celular CyQUANT™ NF proporciona un método rápido y sensible para el recuento de célulasMás información

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Número de catálogo	Tipo de célula	Cantidad
C7026	For cells in culture	1000 ensayos
C35013	Direct Red Cell	10 Microplacas
C35007	NF Cell	200 Assays

3 opciones

Número de catálogo C7026

Precio (USD)

741,96

Each

Añadir al carro de la compra

Tipo de célula:

For cells in culture

Cantidad:

1000 ensayos

Precio (USD)

741,96

Each

Añadir al carro de la compra

El kit de ensayo de proliferación celular CyQUANT™ NF proporciona un método rápido y sensible para el recuento de células en una población y la medición de la proliferación en formato de microplaca.

Características del ensayo de proliferación celular CyQUANT™ NF:

• Más sensible que los ensayos MTT o alamarBlue™
• Rango de detección lineal de 100 a 20.000 células por pocillo (microplaca de 96 pocillos)
• Permite completar los ensayos en una hora

Un método rápido y sencillo para medir la proliferación celular
El ensayo de proliferación celular CyQUANT™ NF no requiere lisis celular, incubaciones largas, radioactividad ni eliminación de tinte de las células. El ensayo CyQUANT™ NF elimina el paso de lisis celular con congelación-descongelación del ensayo de proliferación celular CyQUANT™ original utilizando un tinte fijador de ADN y permeable en célula con un reactivo de permeabilización de la membrana de plasma. El ensayo de proliferación celular CyQUANT™ NF se puede utilizar en cualquiera de los formatos de microplacas de 96 pocillos o 384 pocillos, y está disponible en dos configuraciones: un kit de ensayo 200 (C35007) para pequeños tamaños de muestra y un kit de ensayo 1000 (C35006) para aplicaciones de alto rendimiento.

alamarBlue™ es una marca registrada de TREK Diagnostic Systems.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularImpenetrable en células

Tipo de célulaFor cells in culture

DescripciónEnsayo de proliferación celular CyQUANT™, para células en cultivo

Método de detecciónFluorescente

Tipo de coloranteOtras etiquetas o colorantes

Excitación/emisión480/520

FormatoPlaca de 96 pocillos

Cantidad1000 ensayos

Condiciones de envíoTemperatura ambiente

ColorVerde

Emission520 nm

Excitation Wavelength Range480 nm

Para utilizar con (aplicación)Ensayo de proliferación

Para utilizar con (equipo)Lector de microplacas

Línea de productosCyQUANT

Tipo de productoEnsayo de proliferación celular

Unit SizeEach

Contenido y almacenamiento

Contiene colorante CyQUANT™ GR, tampón de lisis celular y ADN de bacteriófago λ.

Preguntas frecuentes

Can CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026) be used on organoids in Matrigel medium?

CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) has been validated for use with the AlgiMatrix 3D Culture System, but we cannot guarantee that it will work with other 3D culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026)?

The composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture is proprietary.

Which cell proliferation assays can I use with 3D culture systems?

alamarBlue Cell Viability Reagent, PrestoBlue Cell Viability Reagent, and CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) have been validated for use with the AlgiMatrix 3D Culture System and should also work with other 3D culture systems. For further details, please refer to this poster (https://aacrjournals.org/cancerres/article/70/8_Supplement/3234/563757/Abstract-3234-AlgiMatrixT-3-D-cell-culture-system) and protocol (http://www.thermofisher.com/us/en/home/references/protocols/cell-culture/3-d-cell-culture-protocol/algimatrix-viability-using-cyquant.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (65)

Citations & References

Abstract

Growth factor-induced proliferation in corneal epithelial cells is mediated by 12(S)-HETE.

Authors:Ottino P,Taheri F,Bazan HE

Journal:Experimental eye research

PubMed ID:12697425

PURPOSE: Previous studies in our laboratory have shown that 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE), a product of 12-lipoxygenase (12-LOX) activity, is the predominant metabolite formed in rabbit corneas after injury. The present study was undertaken to investigate the effects of epidermal growth factor (EGF), hepatocyte growth factor (HGF), and keratinocyte growth factor ... More

Caspase activation contributes to delayed death of heat-stressed striatal neurons.

Authors:White MG, Emery M, Nonner D, Barrett JN

Journal:J Neurochem

PubMed ID:14622126

'Hyperthermia can contribute to brain damage both during development and post-natally. We used rat embryonic striatal neurons in culture to study mechanisms underlying hyperthermia-induced neuronal death. Heat stress at 43 degrees C for 2 h produced no obvious signs of damage during the first 12 h after the stress, but ... More

Endothelial cell surface F1-F0 ATP synthase is active in ATP synthesis and is inhibited by angiostatin.

Authors:Moser TL, Kenan DJ, Ashley TA, Roy JA, Goodman MD, Misra UK, Cheek DJ, Pizzo SV

Journal:Proc Natl Acad Sci U S A

PubMed ID:11381144

'Angiostatin blocks tumor angiogenesis in vivo, almost certainly through its demonstrated ability to block endothelial cell migration and proliferation. Although the mechanism of angiostatin action remains unknown, identification of F(1)-F(O) ATP synthase as the major angiostatin-binding site on the endothelial cell surface suggests that ATP metabolism may play a role ... More

Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.

Authors:Silva E, Gomes P, Soares-da-Silva P,

Journal:J Membr Biol

PubMed ID:17334838

'Na(+)/K(+)-ATPase plays a key role in the transport of Na(+) throughout the nephron, but ageing appears to be accompanied by changes in the regulation and localization of the pump. In the present study, we examined the effect of in vitro cell ageing on the transport of Na(+) and K(+) ions ... More

Identification of the binding site for fibrinogen recognition peptide gamma 383-395 within the alpha(M)I-domain of integrin alpha(M)beta2.

Authors:Yakubenko VP, Solovjov DA, Zhang L, Yee VC, Plow EF, Ugarova TP

Journal:J Biol Chem

PubMed ID:11278633

'The leukocyte integrin alpha(M)beta(2) (Mac-1, CD11b/CD18) is a cell surface adhesion receptor for fibrinogen. The interaction between fibrinogen and alpha(M)beta(2) mediates a range of adhesive reactions during the immune-inflammatory response. The sequence gamma(383)TMKIIPFNRLTIG(395), P2-C, within the gamma-module of the D-domain of fibrinogen, is a recognition site for alpha(M)beta(2) and alpha(X)beta(2). ... More

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