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Citas y referencias (18)
Invitrogen™
DQ™ Collagen, type IV From Human Placenta, Fluorescein Conjugate
El colágeno fluorogénico DQ™ se puede utilizar para controlar directamente la actividad de colagenasa. Los sustratos DQ™ son análogos delMás información
| Número de catálogo | Cantidad |
|---|---|
| D12052 | 1 mg |
Número de catálogo D12052
Precio (USD)
-
Cantidad:
1 mg
El colágeno fluorogénico DQ™ se puede utilizar para controlar directamente la actividad de colagenasa. Los sustratos DQ™ son análogos del sustrato natural que tienen un número excesivo de tintes fluorescentes adheridos de modo que la señal de fluorescencia es casi inexistente. La proximidad de los tintes sobre el sustrato intacto causa esta supresión de señal. La hidrólisis del sustrato impulsada por enzimas da como resultado la separación del tinte, lo que provoca la separación de las moléculas del tinte entre sí y aumenta la señal de fluorescencia.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de productosDQ
Cantidad1 mg
Condiciones de envíoTemperatura ambiente
SustratoSustrato de proteasa
Método de detecciónFluorescente
FormularioLiofilizado
Substrate PropertiesSustrato basado en proteínas
Target EnzymeMetaloproteinasa
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Citations & References (18)
Citations & References
Abstract
Intracellular and extracellular cathepsin B facilitate invasion of MCF-10A neoT cells through reconstituted extracellular matrix in vitro.
Journal:Exp Cell Res
PubMed ID:12581740
'Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using specific cathepsin
Matrix metalloproteinase activity in urine of patients with renal cell carcinoma leads to degradation of extracellular matrix proteins: possible use as a screening assay.
Journal:J Urol
PubMed ID:12629409
'PURPOSE: Localized renal cell carcinoma is usually curable by nephrectomy. However, a large fraction of patients already present with metastatic disease, which results in a poor outcome. Currently no clinically relevant screening assay is available to detect early stage renal cell carcinoma. We investigated whether urinary extracellular matrix (ECM) proteins
Imaging proteolysis by living human glioma cells.
Journal:Biol Chem
PubMed ID:11517931
'Degradation of basement membrane is an essential step for tumor invasion. In order to study degradation in real time as well as localize the site of proteolysis, we have established an assay with living human cancer cells in which we image cleavage of quenched-fluorescent basement membrane type IV collagen (DQ-collagen
Metabolic mapping of proteinase activity with emphasis on in situ zymography of gelatinases: review and protocols.
Journal:J Histochem Cytochem
PubMed ID:15150280
'Proteases are essential for protein catabolism, regulation of a wide range of biological processes, and in the pathogenesis of many diseases. Several techniques are available to localize activity of proteases in tissue sections or cell preparations. For localization of the activity of matrix metalloproteinases, in situ zymography was introduced some
Functional imaging of proteolysis: stromal and inflammatory cells increase tumor proteolysis.
Journal:Mol Imaging
PubMed ID:14649059
'The underlying basement membrane is degraded during progression of breast and colon carcinoma. Thus, we imaged degradation of a quenched fluorescent derivative of basement membrane type IV collagen (DQ-collagen IV) by living human breast and colon tumor spheroids. Proteolysis of DQ-collagen IV by HCT 116 and HKh-2 human colon tumor